Requirement for GD3 ganglioside in CD95- and ceramide-induced apoptosis

Ruggero De Maria, Luisa Lenti, Florence Malisan, Federica D'Agostino, Barbara Tomassini, Ann Zeuner, Maria Rita Rippo, Roberte Testi

Research output: Contribution to journalArticle

Abstract

Gangliosides participate in development and tissue differentiation. Cross-linking of the apoptosis-inducing CD95 protein (also called Fas or APO- 1) in lymphoid and myeloid tumor cells triggered GD3 ganglioside synthesis and transient accumulation. CD95-induced GD3 accumulation depended on integral receptor 'death domains' and on activation of a family of cysteine proteases called caspases. Cell-permeating ceramides, which are potent inducers of apoptosis, also triggered GD3 synthesis. GD3 disrupted mitochondrial transmembrane potential (ΔΨ(m)), and induced apoptosis, in a caspase-independent fashion. Transient overexpression of the GD3 synthase gene directly triggered apoptosis. Pharmacological inhibition of GD3 synthesis and exposure to GD3 synthase antisense oligodeoxynucleotides prevented CD95-induced apoptosis. Thus, GD3 ganglioside mediates the propagation of CD95-generated apoptotic signals in hematopoietic cells.

Original languageEnglish
Pages (from-to)1652-1655
Number of pages4
JournalScience
Volume277
Issue number5332
DOIs
Publication statusPublished - Sep 12 1997

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Ceramides
Apoptosis
Caspases
Death Domain Receptors
Apoptosis Regulatory Proteins
Cysteine Proteases
Gangliosides
Oligodeoxyribonucleotides
Myeloid Cells
Membrane Potentials
Pharmacology
GD3 ganglioside
Genes
Neoplasms

ASJC Scopus subject areas

  • General

Cite this

De Maria, R., Lenti, L., Malisan, F., D'Agostino, F., Tomassini, B., Zeuner, A., ... Testi, R. (1997). Requirement for GD3 ganglioside in CD95- and ceramide-induced apoptosis. Science, 277(5332), 1652-1655. https://doi.org/10.1126/science.277.5332.1652

Requirement for GD3 ganglioside in CD95- and ceramide-induced apoptosis. / De Maria, Ruggero; Lenti, Luisa; Malisan, Florence; D'Agostino, Federica; Tomassini, Barbara; Zeuner, Ann; Rippo, Maria Rita; Testi, Roberte.

In: Science, Vol. 277, No. 5332, 12.09.1997, p. 1652-1655.

Research output: Contribution to journalArticle

De Maria, R, Lenti, L, Malisan, F, D'Agostino, F, Tomassini, B, Zeuner, A, Rippo, MR & Testi, R 1997, 'Requirement for GD3 ganglioside in CD95- and ceramide-induced apoptosis', Science, vol. 277, no. 5332, pp. 1652-1655. https://doi.org/10.1126/science.277.5332.1652
De Maria R, Lenti L, Malisan F, D'Agostino F, Tomassini B, Zeuner A et al. Requirement for GD3 ganglioside in CD95- and ceramide-induced apoptosis. Science. 1997 Sep 12;277(5332):1652-1655. https://doi.org/10.1126/science.277.5332.1652
De Maria, Ruggero ; Lenti, Luisa ; Malisan, Florence ; D'Agostino, Federica ; Tomassini, Barbara ; Zeuner, Ann ; Rippo, Maria Rita ; Testi, Roberte. / Requirement for GD3 ganglioside in CD95- and ceramide-induced apoptosis. In: Science. 1997 ; Vol. 277, No. 5332. pp. 1652-1655.
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