Requirement for membrane lipid peroxidation in HIV-1 gp120-induced neuroblastoma cell death

M. T. Corasaniti, M. Navarra, S. Nisticò, D. Rotiroti, M. MacCarrone, G. Melino, A. Finazzi-Agrò

Research output: Contribution to journalArticlepeer-review

Abstract

The HIV-1 envelope protein gp120 engenders Ca2+-mediated, excitotoxic damage of rodent neuronal and human neuroblastoma cells in culture. Here we report that human CHP100 neuroblastoma cells undergo early peroxidation of membrane lipids following a brief exposure to gp120. This effect is prevented by preincubating cell cultures with the 21-aminosteroid U-74389G, an inhibitor of membrane lipid peroxidation, which also rescues neuroblastoma cultures from gp120-induced cell death; conversely, no protection from cell death is observed when the 21-aminosteroid is added to neuroblastoma cultures after the induction of membrane lipid peroxidation by gp120. These data indicate that membrane lipid peroxidation has a causative role in the expression of cell death produced by the viral protein.

Original languageEnglish
Pages (from-to)686-689
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume246
Issue number3
DOIs
Publication statusPublished - May 29 1998

Keywords

  • HIV-1 gp120
  • Membrane lipid peroxidation
  • Neuroblastoma cell death
  • U-74389G

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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