Requirement for the histone deacetylase Hdac3 for the inflammatory gene expression program in macrophages

Xuefen Chen, Iros Barozzi, Alberto Termanini, Elena Prosperini, Antonio Recchiuti, Jesmond Dalli, Flore Mietton, Gianluca Matteoli, Scott Hiebert, Gioacchino Natoli

Research output: Contribution to journalArticle

Abstract

Histone deacetylases (HDACs) regulate inflammatory gene expression, as indicated by the potent antiinflammatory activity of pan-HDAC inhibitors. However, the specific contribution of each of the 11 HDAC proteins to the inflammatory gene expression program is unknown. Using an integrated genomic approach, we found that Hdac3-deficient macrophages were unable to activate almost half of the inflammatory gene expression program when stimulated with LPS. A large part of the activation defect was attributable to loss of basal and LPS-inducible expression of IFN-β, which maintains Stat1 protein levels in unstimulated cells and acts in an autocrine/paracrine manner after stimulation to promote a secondary wave of Stat1-dependent gene expression. Loss of Hdac3-mediated repression of nuclear receptors led to hyperacetylation of thousands of genomic sites and associated gene derepression. The up-regulation of the constitutively expressed prostaglandin endoperoxide synthase, Ptgs1 (Cox-1), a nuclear receptor target, had a causative role in the phenotype because its chemical inhibition reverted, albeit partially, the Ifn-β activation defect. These data indicate a central role for Hdac3 in inflammation and may have relevance for the use of selective Hdac inhibitors as antiinflammatory agents.

Original languageEnglish
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number42
DOIs
Publication statusPublished - Oct 16 2012

Keywords

  • Chromatin
  • Transcription

ASJC Scopus subject areas

  • General

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