Requirement for the ryanodine receptor type 3 for efficient contraction in neonatal skeletal muscles

Federica Bertocchini, Catherine E. Ovitt, Antonio Conti, Virginia Barone, Hans R. Schöler, Roberto Bottinelli, Carlo Reggiani, Vincenzo Sorrentino

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

The skeletal isoform of Ca2+ release channel, RyR1, plays a central role in activation of skeletal muscle contraction. Another isoform, RyR3, has been observed recently in some mammalian skeletal muscles, but whether it participates in regulating skeletal muscle contraction is not known. The expression of RyR3 in skeletal muscles was studied in mice from late fetal stages to adult life. RyR3 was found to be expressed widely in murine skeletal muscles during the post-natal phase of muscle development, but was not detectable in muscles of adult mice, with the exception of the diaphragm and soleus muscles. RyR3 knockout mice were generated, and it was shown that skeletal muscle contraction in these mice was impaired during the first weeks after birth. In skeletal muscles isolated from newborn RyR3(-/-) mice, but not in those from adult mice, the twitch elicited by electrical stimulation and the contracture induced by caffeine were strongly depressed. These results provide the first evidence that RyR3 has a physiological role in excitation-contraction coupling of neonatal skeletal muscles. The disproportion between the low amount of RyR3 and the large impact of the RyR3 knockout suggests that this isoform contributes to the amplification of Ca2+ released by the existing population of ryanodine receptors (RyR1).

Original languageEnglish
Pages (from-to)6956-6963
Number of pages8
JournalEMBO Journal
Volume16
Issue number23
Publication statusPublished - Dec 1 1997

Fingerprint

Ryanodine Receptor Calcium Release Channel
Muscle
Skeletal Muscle
Muscle Contraction
Protein Isoforms
Excitation Contraction Coupling
Muscle Development
Contracture
Diaphragms
Diaphragm
Caffeine
Knockout Mice
Electric Stimulation
Amplification
Chemical activation
Parturition

Keywords

  • Ca release channels
  • Muscle contraction
  • Muscle development
  • Ryanodine receptors

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Bertocchini, F., Ovitt, C. E., Conti, A., Barone, V., Schöler, H. R., Bottinelli, R., ... Sorrentino, V. (1997). Requirement for the ryanodine receptor type 3 for efficient contraction in neonatal skeletal muscles. EMBO Journal, 16(23), 6956-6963.

Requirement for the ryanodine receptor type 3 for efficient contraction in neonatal skeletal muscles. / Bertocchini, Federica; Ovitt, Catherine E.; Conti, Antonio; Barone, Virginia; Schöler, Hans R.; Bottinelli, Roberto; Reggiani, Carlo; Sorrentino, Vincenzo.

In: EMBO Journal, Vol. 16, No. 23, 01.12.1997, p. 6956-6963.

Research output: Contribution to journalArticle

Bertocchini, F, Ovitt, CE, Conti, A, Barone, V, Schöler, HR, Bottinelli, R, Reggiani, C & Sorrentino, V 1997, 'Requirement for the ryanodine receptor type 3 for efficient contraction in neonatal skeletal muscles', EMBO Journal, vol. 16, no. 23, pp. 6956-6963.
Bertocchini F, Ovitt CE, Conti A, Barone V, Schöler HR, Bottinelli R et al. Requirement for the ryanodine receptor type 3 for efficient contraction in neonatal skeletal muscles. EMBO Journal. 1997 Dec 1;16(23):6956-6963.
Bertocchini, Federica ; Ovitt, Catherine E. ; Conti, Antonio ; Barone, Virginia ; Schöler, Hans R. ; Bottinelli, Roberto ; Reggiani, Carlo ; Sorrentino, Vincenzo. / Requirement for the ryanodine receptor type 3 for efficient contraction in neonatal skeletal muscles. In: EMBO Journal. 1997 ; Vol. 16, No. 23. pp. 6956-6963.
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