Requirement of Pyk2 for the activation of the MAP kinase cascade induced by Ca2+ (but not by PKC or G protein) in PC12 cells

Rico Barsacchi, Harald Heider, Jean Antoine Girault, Jacopo Meldolesi

Research output: Contribution to journalArticlepeer-review

Abstract

The role of the Ca2+-activated tyrosine kinase, Pyk2, in the pleiotropic coupling of nerve cell stimulation to the MAP kinase cascade still remains undefined. Using a panel of PC12 clones, one of which was defective in Pyk2, we demonstrate (1) that the MAP kinase response induced by a [Ca2+](i) rise (following application of the Ca2+ ionophore, ionomycin) is inappreciable in the defective clone and is re-established after Pyk2 transfection; and (2) that the responses to both protein kinase C and P(2y2) receptor activation occur normally even in the defective cells. We conclude that Pyk2 is the key mediator in the pathway activated by Ca2+ but has minor roles with the other types of stimulation. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)273-276
Number of pages4
JournalFEBS Letters
Volume461
Issue number3
DOIs
Publication statusPublished - Nov 19 1999

Keywords

  • Calcium
  • Mitogen-activated protein kinase
  • P(2y2) receptor
  • PC12
  • Pyk2
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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