Abstract
Dense-core granules (DCGs), cytoplasmic organelles competent for regulated exocytosis, show considerable heterogeneity depending upon the specificity of their expressing cells - primarily neurons and neurosecretory cells. DCGs have been mainly identified by detecting their cargo molecules, often members of the granin family, and using conventional electron microscopy and immunocytochemistry. However, by a critical analysis of the various stages of DCG 'life' within neurosecretory cells, we have highlighted several specific molecular and functional properties that are common to all these organelles. We propose that these properties be considered as strict requirements for the identification of DCGs.
| Original language | English |
|---|---|
| Pages (from-to) | 13-19 |
| Number of pages | 7 |
| Journal | Trends in Cell Biology |
| Volume | 14 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2004 |
Fingerprint
ASJC Scopus subject areas
- Cell Biology
Cite this
Requirements for the identification of dense-core granules. / Meldolesi, Jacopo; Chieregatti, Evelina; Malosio, Maria Luisa.
In: Trends in Cell Biology, Vol. 14, No. 1, 01.2004, p. 13-19.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Requirements for the identification of dense-core granules
AU - Meldolesi, Jacopo
AU - Chieregatti, Evelina
AU - Malosio, Maria Luisa
PY - 2004/1
Y1 - 2004/1
N2 - Dense-core granules (DCGs), cytoplasmic organelles competent for regulated exocytosis, show considerable heterogeneity depending upon the specificity of their expressing cells - primarily neurons and neurosecretory cells. DCGs have been mainly identified by detecting their cargo molecules, often members of the granin family, and using conventional electron microscopy and immunocytochemistry. However, by a critical analysis of the various stages of DCG 'life' within neurosecretory cells, we have highlighted several specific molecular and functional properties that are common to all these organelles. We propose that these properties be considered as strict requirements for the identification of DCGs.
AB - Dense-core granules (DCGs), cytoplasmic organelles competent for regulated exocytosis, show considerable heterogeneity depending upon the specificity of their expressing cells - primarily neurons and neurosecretory cells. DCGs have been mainly identified by detecting their cargo molecules, often members of the granin family, and using conventional electron microscopy and immunocytochemistry. However, by a critical analysis of the various stages of DCG 'life' within neurosecretory cells, we have highlighted several specific molecular and functional properties that are common to all these organelles. We propose that these properties be considered as strict requirements for the identification of DCGs.
UR - http://www.scopus.com/inward/record.url?scp=0347915585&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347915585&partnerID=8YFLogxK
U2 - 10.1016/j.tcb.2003.11.006
DO - 10.1016/j.tcb.2003.11.006
M3 - Article
C2 - 14729176
AN - SCOPUS:0347915585
VL - 14
SP - 13
EP - 19
JO - Trends in Cell Biology
JF - Trends in Cell Biology
SN - 0962-8924
IS - 1
ER -