Rescue of impaired angiogenesis in spontaneously hypertensive rats by intramuscular human tissue kallikrein gene transfer

Costanza Emanueli, Maria Bonaria Salis, Tiziana Stacca, Leonardo Gaspa, Julie Chao, Lee Chao, Andrea Piana, Paolo Madeddu

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Angiogenesis represents a compensatory response targeted to preserve the integrity of tissues subjected to ischemia. The aim of the present study was to examine whether reparative angiogenesis is impaired in spontaneously hypertensive rats (SHR), as a function of progression of hypertension. In addition, the potential of gene therapy with human tissue kallikrein (HK) in revascularization was challenged in SHR and normotensive Wistar-Kyoto rats (WKY) that underwent excision of the left femoral artery. Expression of vascular endothelial growth factor and HK was upregulated in ischemic hindlimb of WKY but not of SHR. Capillary density was increased in ischemic adductor muscle of WKY (from 266±20 to 633±73 capillaries/mm2 at 28 days, P2, P=NS), thus compromising perfusion recovery as indicated by reduced plantar blood flow ratio (0.61±0.08 versus 0.92±0.07 in WKY at 28 days, P9 pfu adenovirus containing the HK gene (Ad.CMV-cHK) or the β-galactosidase gene (Ad.CMV-LacZ) was injected intramuscularly at 7 days after the induction of ischemia. Ad.CMV-cHK augmented capillary density and accelerated hemodynamic recovery in both strains, but these effects were more pronounced in SHR (P

Original languageEnglish
Pages (from-to)136-141
Number of pages6
JournalHypertension
Volume38
Issue number1
Publication statusPublished - 2001

Fingerprint

Tissue Kallikreins
Inbred WKY Rats
Inbred SHR Rats
Genes
Ischemia
Galactosidases
Human Adenoviruses
Femoral Artery
Hindlimb
Genetic Therapy
Perfusion
Hemodynamics
Hypertension
Muscles

Keywords

  • Angiogenesis
  • Genes
  • Ischemia
  • Kallikrein
  • Muscles

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Rescue of impaired angiogenesis in spontaneously hypertensive rats by intramuscular human tissue kallikrein gene transfer. / Emanueli, Costanza; Salis, Maria Bonaria; Stacca, Tiziana; Gaspa, Leonardo; Chao, Julie; Chao, Lee; Piana, Andrea; Madeddu, Paolo.

In: Hypertension, Vol. 38, No. 1, 2001, p. 136-141.

Research output: Contribution to journalArticle

Emanueli, C, Salis, MB, Stacca, T, Gaspa, L, Chao, J, Chao, L, Piana, A & Madeddu, P 2001, 'Rescue of impaired angiogenesis in spontaneously hypertensive rats by intramuscular human tissue kallikrein gene transfer', Hypertension, vol. 38, no. 1, pp. 136-141.
Emanueli, Costanza ; Salis, Maria Bonaria ; Stacca, Tiziana ; Gaspa, Leonardo ; Chao, Julie ; Chao, Lee ; Piana, Andrea ; Madeddu, Paolo. / Rescue of impaired angiogenesis in spontaneously hypertensive rats by intramuscular human tissue kallikrein gene transfer. In: Hypertension. 2001 ; Vol. 38, No. 1. pp. 136-141.
@article{0d4f267eb9cb428f876db5ff369ee514,
title = "Rescue of impaired angiogenesis in spontaneously hypertensive rats by intramuscular human tissue kallikrein gene transfer",
abstract = "Angiogenesis represents a compensatory response targeted to preserve the integrity of tissues subjected to ischemia. The aim of the present study was to examine whether reparative angiogenesis is impaired in spontaneously hypertensive rats (SHR), as a function of progression of hypertension. In addition, the potential of gene therapy with human tissue kallikrein (HK) in revascularization was challenged in SHR and normotensive Wistar-Kyoto rats (WKY) that underwent excision of the left femoral artery. Expression of vascular endothelial growth factor and HK was upregulated in ischemic hindlimb of WKY but not of SHR. Capillary density was increased in ischemic adductor muscle of WKY (from 266±20 to 633±73 capillaries/mm2 at 28 days, P2, P=NS), thus compromising perfusion recovery as indicated by reduced plantar blood flow ratio (0.61±0.08 versus 0.92±0.07 in WKY at 28 days, P9 pfu adenovirus containing the HK gene (Ad.CMV-cHK) or the β-galactosidase gene (Ad.CMV-LacZ) was injected intramuscularly at 7 days after the induction of ischemia. Ad.CMV-cHK augmented capillary density and accelerated hemodynamic recovery in both strains, but these effects were more pronounced in SHR (P",
keywords = "Angiogenesis, Genes, Ischemia, Kallikrein, Muscles",
author = "Costanza Emanueli and Salis, {Maria Bonaria} and Tiziana Stacca and Leonardo Gaspa and Julie Chao and Lee Chao and Andrea Piana and Paolo Madeddu",
year = "2001",
language = "English",
volume = "38",
pages = "136--141",
journal = "Hypertension",
issn = "0194-911X",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Rescue of impaired angiogenesis in spontaneously hypertensive rats by intramuscular human tissue kallikrein gene transfer

AU - Emanueli, Costanza

AU - Salis, Maria Bonaria

AU - Stacca, Tiziana

AU - Gaspa, Leonardo

AU - Chao, Julie

AU - Chao, Lee

AU - Piana, Andrea

AU - Madeddu, Paolo

PY - 2001

Y1 - 2001

N2 - Angiogenesis represents a compensatory response targeted to preserve the integrity of tissues subjected to ischemia. The aim of the present study was to examine whether reparative angiogenesis is impaired in spontaneously hypertensive rats (SHR), as a function of progression of hypertension. In addition, the potential of gene therapy with human tissue kallikrein (HK) in revascularization was challenged in SHR and normotensive Wistar-Kyoto rats (WKY) that underwent excision of the left femoral artery. Expression of vascular endothelial growth factor and HK was upregulated in ischemic hindlimb of WKY but not of SHR. Capillary density was increased in ischemic adductor muscle of WKY (from 266±20 to 633±73 capillaries/mm2 at 28 days, P2, P=NS), thus compromising perfusion recovery as indicated by reduced plantar blood flow ratio (0.61±0.08 versus 0.92±0.07 in WKY at 28 days, P9 pfu adenovirus containing the HK gene (Ad.CMV-cHK) or the β-galactosidase gene (Ad.CMV-LacZ) was injected intramuscularly at 7 days after the induction of ischemia. Ad.CMV-cHK augmented capillary density and accelerated hemodynamic recovery in both strains, but these effects were more pronounced in SHR (P

AB - Angiogenesis represents a compensatory response targeted to preserve the integrity of tissues subjected to ischemia. The aim of the present study was to examine whether reparative angiogenesis is impaired in spontaneously hypertensive rats (SHR), as a function of progression of hypertension. In addition, the potential of gene therapy with human tissue kallikrein (HK) in revascularization was challenged in SHR and normotensive Wistar-Kyoto rats (WKY) that underwent excision of the left femoral artery. Expression of vascular endothelial growth factor and HK was upregulated in ischemic hindlimb of WKY but not of SHR. Capillary density was increased in ischemic adductor muscle of WKY (from 266±20 to 633±73 capillaries/mm2 at 28 days, P2, P=NS), thus compromising perfusion recovery as indicated by reduced plantar blood flow ratio (0.61±0.08 versus 0.92±0.07 in WKY at 28 days, P9 pfu adenovirus containing the HK gene (Ad.CMV-cHK) or the β-galactosidase gene (Ad.CMV-LacZ) was injected intramuscularly at 7 days after the induction of ischemia. Ad.CMV-cHK augmented capillary density and accelerated hemodynamic recovery in both strains, but these effects were more pronounced in SHR (P

KW - Angiogenesis

KW - Genes

KW - Ischemia

KW - Kallikrein

KW - Muscles

UR - http://www.scopus.com/inward/record.url?scp=0035569052&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035569052&partnerID=8YFLogxK

M3 - Article

C2 - 11463774

AN - SCOPUS:0035569052

VL - 38

SP - 136

EP - 141

JO - Hypertension

JF - Hypertension

SN - 0194-911X

IS - 1

ER -