Abstract
The levels of benzo(a)pyrene diolepoxide(BPDE)-DNA adducts were measured in rat and human cells by synchronous fluorescence spectrophotometry. BPDE-DNA adducts detected in human pulmonary alveolar macrophages were related to current smoking habits, in contrast to the adducts found in peripheral blood leukocytes. BP administration to rats produced BPDE-DNA adducts in both liver and lungs. Although small yet repeatable signals were also detected in lung DNA from rats treated for 3 days with tobacco smoke. None of the samples obtained from untreated animals was positive. The detection of BPDE-DNA adducts may be used in biomonitoring and experimental studies for determining of exposure to BP even when applied as a constituent of complex mixtures.
| Translated title of the contribution | Research on adducts of benzo(a)pyrene and DNA in different cells using synchronous fluorescence spectrophotometry |
|---|---|
| Original language | Italian |
| Pages (from-to) | 869-874 |
| Number of pages | 6 |
| Journal | Bollettino della Societa Italiana di Biologia Sperimentale |
| Volume | 67 |
| Issue number | 9 |
| Publication status | Published - Sep 1991 |
ASJC Scopus subject areas
- Medicine(all)