Abstract
Background: Residual telomerase activity (TA) could be used as a marker of malignant or normal cell survival after exposure to cytotoxic agents. Therefore TA after treatment with ionizing radiation was used in a radiosensitivity assay. Materials and Methods: Radiosensitive MOLT-4 and relatively radioresistant Jurkat leukemia cells of normal peripheral blood mononuclear cells (MNC) were irradiated with 5-160 Gy. Cell count, MTT assay and telomerase activity were evaluated on day 3 and clonogenic assay on day 10. Results: In Jurkat cells, irradiation inhibited tumor growth and total TA per culture (TA/culture), but up-regulated TA per viable cell (TA/cell). TA/culture and TA/cell were profoundly depressed by irradiation of MOLT-4 or phytohemagglutinin-activated MNC. Conclusion: Susceptibility of normal or neoplastic cells to high-dose radiation can be monitored by evaluating residual TA/culture. In some cases, however, difficulties in the interpretation of the results could stem from radiation-induced increase of TA/cell, as observed for the Jurkat cell line.
Original language | English |
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Pages (from-to) | 4561-4569 |
Number of pages | 9 |
Journal | Anticancer Research |
Volume | 23 |
Issue number | 6 C |
Publication status | Published - Nov 2003 |
Keywords
- Lymphocytes
- Oncology
- Radiation
- Radiosensitivity
- Telomerase
ASJC Scopus subject areas
- Cancer Research
- Oncology