Resiquimod-Mediated Activation of Plasmacytoid Dendritic Cells Is Amplified in Multiple Sclerosis

Marta Corsetti, Gabriella Ruocco, Serena Ruggieri, Claudio Gasperini, Luca Battistini, Elisabetta Volpe

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease of the central nervous system. The cause of multiple sclerosis is unknown but there are several evidences that associate the genetic basis of the disease with environmental causes. An important association between viral infection and development of MS is clearly demonstrated. Viruses have a strong impact on innate immune cells. In particular, myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs), are able to respond to viruses and to activate the adaptive immune response. METHODS: In this study we mimic viral infection using synthetic single-strand RNA, Resiquimod, and we compared the response of both DC subsets derived from healthy donors and MS patients by characterizing the expression of costimulatory molecules on the DC surface. RESULTS: We found that pDCs from MS patients express higher levels of OX40-L, HLA-DR, and CD86 than healthy donors. Moreover, we found that blood cells from MS patients and healthy donors upon Resiquimod-stimulation are enriched in a subpopulation of pDCs, characterized by a high amount of costimulatory molecules. CONCLUSION: Overall, these results indicate that activation of pDCs is enhanced in MS, likely due to a latent viral infection, and that costimulatory molecules expressed on pDCs could mediate a protective response against the viral trigger of autoimmunity.

Original languageEnglish
JournalInternational Journal of Molecular Sciences
Volume20
Issue number11
DOIs
Publication statusPublished - Jun 8 2019

Fingerprint

resiquimod
Dendritic Cells
Multiple Sclerosis
Chemical activation
activation
infectious diseases
cells
Virus Diseases
viruses
Viruses
Tissue Donors
Molecules
direct current
Autoimmune Diseases of the Nervous System
molecules
blood cells
central nervous system
causes
Neurology
HLA-DR Antigens

Keywords

  • costimulatory molecules
  • multiple sclerosis
  • myeloid dendritic cells
  • plasmacytoid dendritic cells
  • Resiquimod
  • Toll-like receptors
  • virus infection

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Resiquimod-Mediated Activation of Plasmacytoid Dendritic Cells Is Amplified in Multiple Sclerosis. / Corsetti, Marta; Ruocco, Gabriella; Ruggieri, Serena; Gasperini, Claudio; Battistini, Luca; Volpe, Elisabetta.

In: International Journal of Molecular Sciences, Vol. 20, No. 11, 08.06.2019.

Research output: Contribution to journalArticle

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AU - Volpe, Elisabetta

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