Resistance to Fas-mediated apoptosis of human T-cell lines expressing human T-lymphotropic virus type-2 (HTLV-2) tax protein

Gianguglielmo Zehender, Stefania Varchetta, Chiara De Maddalena, Chiara Colasante, Agostino Riva, Luca Meroni, Mauro Moroni, Massimo Galli

Research output: Contribution to journalArticle

Abstract

The susceptibility to Fas-mediated apoptosis was evaluated in seven T-cell lines (two infected with HTLV-2, one with HTLV-1, and four HTLV-free) as well as in Jurkat cells transfected with a Tax-2 expressing vector. Fas-mediated apoptosis was significantly reduced in the HTLV-1- and HTLV-2-infected lines in comparison with the HTLV-free lines regardless of the surface expression of Fas antigen (which was no different in the infected and uninfected cells). Fas-mediated apoptosis was also significantly inhibited in Jurkat cells transfected with the Tax-2 expressing vector without any modification in Fas expression. There was significantly more antiapoptotic Bcl-xL mRNA and protein in the transfected than in the untransfected Jurkat T cells. In conclusion, our results suggest that HTLV-2 is capable of inhibiting Fas-mediated apoptosis by means of a mechanism involving the tax-2 gene and probably the expression of bcl-xL messenger and protein.

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalVirology
Volume281
Issue number1
DOIs
Publication statusPublished - Mar 1 2001

Keywords

  • Bcl-X
  • HTLV-2 pathogenesis
  • Human retroviruses
  • Programmed cell death
  • Tax protein

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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