TY - JOUR
T1 - Resolution of HLA-B*44
T2 - 02:01G, -DRB1* 14:01:01G and -DQB1*03:01:01G reveals a high allelic variability among 12 European populations
AU - Vidan-Jeras, B.
AU - Buhler, S.
AU - Dubois, V.
AU - Grubic, Z.
AU - Ivanova, M.
AU - Jaatinen, T.
AU - Ligeiro, D.
AU - Lokki, M. L.
AU - Papasteriades, C.
AU - Poli, F.
AU - Spyropoulou-Vlachou, M.
AU - Tordai, A.
AU - Viken, M. K.
AU - Wenda, S.
AU - Nunes, J. M.
AU - Sanchez-Mazas, A.
AU - Tiercy, Jean Marie
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Within the framework of the EU-funded HLA-NET action, an analysis of three G-group alleles, HLA-B* 44:02:01G, DRB1* 14:01:01G and DQB1* 03:01:01G, was undertaken in 12 European populations. Ambiguities were resolved by polymerase chain reaction-sequence-specific amplification (PCR-SSP) or PCR-sequence-based typing (PCR-SBT) in a total of 5095 individuals. The results of the DRB1* 14:01/14:54 ambiguity showed high relative ratios (24-53%) of DRB1* 14:01 in Bulgarians, Croatians, Greeks, Italians and Slovenians, contrasting with low ratios (6-13%) in Austrians, Finnish, French, Hungarians, Norwegians and Swiss. Resolution of the B* 44:02/44:27 ambiguity showed that B* 44:27 had a high relative ratio in Slovenians (25.5%) and Bulgarians (37%) and low in French and Swiss (0.02-1%), and was not observed in Greeks and Italians. The highest relative ratio of DQB1* 03:19 was found in Portuguese (11%), by contrast with low ratios (0-3%) in the other five populations. Analysis of the A, B, DRB1* phenotypes and family-derived haplotypes in 1719 and 403 individuals positive for either HLA-B* 44:02G or DRB1* 14:01G ambiguities, respectively, showed some preferential associations, such as A* 26~ DRB1* 14:01, B* 35~ DRB1* 14:01, B* 38~ DRB1* 14:01 and B* 44:27~ DRB1* 16. Because these ambiguities are located outside the peptide-binding site, they may not be recognized by alloreactive T-cells. However, because of strong linkage disequilibrium (LD), the DRB1* 14:01 vs DRB1* 14:54 and the B* 44:02 vs B* 44:27 mismatches are associated to DRB3-, and C-mismatches, respectively. These results are informative for algorithms searching unrelated hematopoietic stem cell donors. For B* 44:27-positive patients, searches are expected to be more successful when requesting donors from Southeastern-European ancestry. Furthermore, the introduction of human leukocyte antigen (HLA)-typing strategies that allow resolving exon 4 (for class I) and exon 3 (for class II) polymorphisms can be expected to contribute significantly to population genetics studies.
AB - Within the framework of the EU-funded HLA-NET action, an analysis of three G-group alleles, HLA-B* 44:02:01G, DRB1* 14:01:01G and DQB1* 03:01:01G, was undertaken in 12 European populations. Ambiguities were resolved by polymerase chain reaction-sequence-specific amplification (PCR-SSP) or PCR-sequence-based typing (PCR-SBT) in a total of 5095 individuals. The results of the DRB1* 14:01/14:54 ambiguity showed high relative ratios (24-53%) of DRB1* 14:01 in Bulgarians, Croatians, Greeks, Italians and Slovenians, contrasting with low ratios (6-13%) in Austrians, Finnish, French, Hungarians, Norwegians and Swiss. Resolution of the B* 44:02/44:27 ambiguity showed that B* 44:27 had a high relative ratio in Slovenians (25.5%) and Bulgarians (37%) and low in French and Swiss (0.02-1%), and was not observed in Greeks and Italians. The highest relative ratio of DQB1* 03:19 was found in Portuguese (11%), by contrast with low ratios (0-3%) in the other five populations. Analysis of the A, B, DRB1* phenotypes and family-derived haplotypes in 1719 and 403 individuals positive for either HLA-B* 44:02G or DRB1* 14:01G ambiguities, respectively, showed some preferential associations, such as A* 26~ DRB1* 14:01, B* 35~ DRB1* 14:01, B* 38~ DRB1* 14:01 and B* 44:27~ DRB1* 16. Because these ambiguities are located outside the peptide-binding site, they may not be recognized by alloreactive T-cells. However, because of strong linkage disequilibrium (LD), the DRB1* 14:01 vs DRB1* 14:54 and the B* 44:02 vs B* 44:27 mismatches are associated to DRB3-, and C-mismatches, respectively. These results are informative for algorithms searching unrelated hematopoietic stem cell donors. For B* 44:27-positive patients, searches are expected to be more successful when requesting donors from Southeastern-European ancestry. Furthermore, the introduction of human leukocyte antigen (HLA)-typing strategies that allow resolving exon 4 (for class I) and exon 3 (for class II) polymorphisms can be expected to contribute significantly to population genetics studies.
KW - B44:02/44:27
KW - DRB1 14:01/14:54
KW - European populations
KW - Human leukocyte antigen ambiguities
KW - Human leukocyte antigen incompatibilities
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U2 - 10.1111/tan.12422
DO - 10.1111/tan.12422
M3 - Article
C2 - 25209151
AN - SCOPUS:84933498100
VL - 84
SP - 459
EP - 464
JO - Tissue Antigens
JF - Tissue Antigens
SN - 0001-2815
IS - 5
ER -