Response of human platelets exposed to arachidonate upon stimulation with other agonists

Research output: Contribution to journalArticle

Abstract

Arachidonate (25 μM) induced only 20% maximal platelet aggregation but 80% maximal cytoplasmic ionized calcium movement (as measured by the photoprotein aequorin). Preincubation with 25 μM arachidonate prevented platelet aggregation and cytoplasmic ionized calcium movement induced by threshold concentrations of the thromboxane A2 analogue U-46619, but did not prevent platelet aggregation induced by threshold concentrations of thrombin, despite a marked reduction of cytoplasmic ionized calcium movement. These findings suggest that thrombin may employ pools of cytoplasmic ionized calcium and/or mechanisms to mobilize it different from those utilized by arachidonate and its metabolites.
Original languageItalian
Pages (from-to)1193-1199
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume149
Issue number3
DOIs
Publication statusPublished - Dec 31 1987

Cite this

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title = "Response of human platelets exposed to arachidonate upon stimulation with other agonists",
abstract = "Arachidonate (25 μM) induced only 20{\%} maximal platelet aggregation but 80{\%} maximal cytoplasmic ionized calcium movement (as measured by the photoprotein aequorin). Preincubation with 25 μM arachidonate prevented platelet aggregation and cytoplasmic ionized calcium movement induced by threshold concentrations of the thromboxane A2 analogue U-46619, but did not prevent platelet aggregation induced by threshold concentrations of thrombin, despite a marked reduction of cytoplasmic ionized calcium movement. These findings suggest that thrombin may employ pools of cytoplasmic ionized calcium and/or mechanisms to mobilize it different from those utilized by arachidonate and its metabolites.",
author = "Chen, {Z. M.} and C. Cerletti and {de Gaetano}, G. and V. Bertel{\'e}",
year = "1987",
month = "12",
day = "31",
doi = "10.1016/0006-291X(87)90534-1",
language = "Italian",
volume = "149",
pages = "1193--1199",
journal = "Biochemical and Biophysical Research Communications",
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publisher = "Academic Press Inc.",
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TY - JOUR

T1 - Response of human platelets exposed to arachidonate upon stimulation with other agonists

AU - Chen, Z. M.

AU - Cerletti, C.

AU - de Gaetano, G.

AU - Bertelé, V.

PY - 1987/12/31

Y1 - 1987/12/31

N2 - Arachidonate (25 μM) induced only 20% maximal platelet aggregation but 80% maximal cytoplasmic ionized calcium movement (as measured by the photoprotein aequorin). Preincubation with 25 μM arachidonate prevented platelet aggregation and cytoplasmic ionized calcium movement induced by threshold concentrations of the thromboxane A2 analogue U-46619, but did not prevent platelet aggregation induced by threshold concentrations of thrombin, despite a marked reduction of cytoplasmic ionized calcium movement. These findings suggest that thrombin may employ pools of cytoplasmic ionized calcium and/or mechanisms to mobilize it different from those utilized by arachidonate and its metabolites.

AB - Arachidonate (25 μM) induced only 20% maximal platelet aggregation but 80% maximal cytoplasmic ionized calcium movement (as measured by the photoprotein aequorin). Preincubation with 25 μM arachidonate prevented platelet aggregation and cytoplasmic ionized calcium movement induced by threshold concentrations of the thromboxane A2 analogue U-46619, but did not prevent platelet aggregation induced by threshold concentrations of thrombin, despite a marked reduction of cytoplasmic ionized calcium movement. These findings suggest that thrombin may employ pools of cytoplasmic ionized calcium and/or mechanisms to mobilize it different from those utilized by arachidonate and its metabolites.

U2 - 10.1016/0006-291X(87)90534-1

DO - 10.1016/0006-291X(87)90534-1

M3 - Articolo

VL - 149

SP - 1193

EP - 1199

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -