Response to caffeine and ryanodine receptor isoforms in mouse skeletal muscles

R. Rossi, R. Bottinelli, V. Sorrentino, C. Reggiani

Research output: Contribution to journalArticlepeer-review


The response to caffeine was studied in mouse muscles [diaphragm, soleus, and extensor digitorum longus (EDL)] with different ryanodine receptor isoform (RyR1, RyR3) composition and in single permeabilized muscle fibers dissected from diaphragm of wild-type (WT) and RyR3-deficient (RyR3-/-) mice at 1, 15, 30, and 60 postnatal days (PND). The caffeine response decreased during development, and, in adult mice, was greater in diaphragm, lower in EDL, and intermediate in soleus. This suggests a direct relation between response to caffeine and RyR3 expression. The lack of RyR3 reduced caffeine response in young, but not in adult mice, and did not abolish the age-dependent variation and the intermuscle differences. In diaphragm single fibers, the response to caffeine increased during development and was reduced in fibers lacking RyR3 both at 15 and 60 PND. A population of fibers highly responsive to caffeine was present in adult WT and disappeared in RyR3-/-. The results confirm the contribution of RyR3 to calcium release for contractile response and clarify the contribution of RyR3 to developmental changes and intermuscle differences.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Issue number2 50-2
Publication statusPublished - 2001


  • Caffeine
  • Calcium release
  • Contractile response
  • Developmental changes
  • Intermuscle differences

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology
  • Physiology (medical)


Dive into the research topics of 'Response to caffeine and ryanodine receptor isoforms in mouse skeletal muscles'. Together they form a unique fingerprint.

Cite this