Responses to sulfated steroids of female mouse vomeronasal sensory neurons

Fulvio Celsi, Anna D'errico, Anna Menini

Research output: Contribution to journalArticle

Abstract

The rodent vomeronasal organ plays an important role in many social behaviors. Using the calcium imaging technique with the dye fluo-4 we measured intracellular calcium concentration changes induced by the application of sulfated steroids to neurons isolated from the vomeronasal organ of female mice. We found that a mix of 10 sulfated steroids from the androgen, estrogen, pregnanolone, and glucocorticoid families induced a calcium response in 71% of neurons. Moreover, 31% of the neurons responded to a mix composed of 3 glucocorticoid-derived compounds, and 28% responded to a mix composed of 3 pregnanolone-derived compounds. Immunohistochemistry showed that neurons responding to sulfated steroids expressed phosphodiesterase 4A, a marker specific for apical neurons expressing V1R receptors. None of the neuron that responded to 1 mix responded also to the other, indicating a specificity of the responses. Some neurons responded to more than 1 individual component of the glucocorticoid-derived mix tested at high concentration, suggesting that these neurons are broadly tuned, although they still displayed strong specificity, remaining unresponsive to high concentrations of the ineffective compounds.

Original languageEnglish
Pages (from-to)849-858
Number of pages10
JournalChemical Senses
Volume37
Issue number9
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Sensory Receptor Cells
Steroids
Neurons
Vomeronasal Organ
Pregnanolone
Glucocorticoids
Calcium
Type 4 Cyclic Nucleotide Phosphodiesterase
Social Behavior
Androgens
Rodentia
Estrogens
Coloring Agents
Immunohistochemistry

Keywords

  • Calcium imaging
  • Odor coding
  • Olfaction
  • Vomeronasal organ

ASJC Scopus subject areas

  • Sensory Systems
  • Behavioral Neuroscience
  • Physiology
  • Physiology (medical)

Cite this

Responses to sulfated steroids of female mouse vomeronasal sensory neurons. / Celsi, Fulvio; D'errico, Anna; Menini, Anna.

In: Chemical Senses, Vol. 37, No. 9, 11.2012, p. 849-858.

Research output: Contribution to journalArticle

Celsi, Fulvio ; D'errico, Anna ; Menini, Anna. / Responses to sulfated steroids of female mouse vomeronasal sensory neurons. In: Chemical Senses. 2012 ; Vol. 37, No. 9. pp. 849-858.
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