TY - JOUR
T1 - Responsiveness of urinary markers of bone resorption to orchiectomy and clodronate treatment in mature rats
T2 - A comparative study
AU - Rubinacci, Alessandro
AU - Villa, Isabella
AU - Sibilia, Valeria
AU - Netti, Carmela
AU - Moro, Gianluigi
AU - Pecile, Antonio
PY - 1998/1
Y1 - 1998/1
N2 - Objective: The aim of this study was to assess the responsiveness of two classes of bone resorption markers to the enhancement of osteoclastic activity induced by orchiectomy and to its inhibition by clodronate treatment in mature rats. Design: Bone mineral density (BMD) at femural metaphysis, femural diaphysis, lumbar vertebrae, and the urinary excretion of pyridinoline (Pyr), deoxypyridinoline (D-Pyr), galactosylhydroxylysine (GHyl) and glucosylgalactosylhydroxylysine (GGHyl) were monitored at regular intervals for 30 days prior to and for 60 days following orchiectomy in eleven rats, divided into two groups: five rats untreated and the other six treated with clodronate. Results: Prior to orchiectomy, a significant (P <0.01) decrease in BMD was observed only at the distal femural metaphysis. This decrease appeared to be associated with a time-dependent increase in the urinary excretion of all markers. Following orchiectomy, the BMD of the untreated group decreased significantly (P <0.01) at all bone sites. The bone loss was accompanied by a significant (P <0.01) increase in Pyr and D- Pyr concentrations in urine, whereas urinary GHyl and GGHyl did not change significantly. In the clodronate-treated group, the BMD of the three skeletal sites did not change significantly, while the urinary excretion of all urinary biochemical markers decreased significantly (P <0.001). Conclusion: This study showed that pyridinolines are able to monitor the bone response to orchiectomy and to clodronate treatment response in androgen-deficient mature male rat, whereas glycosides appear prone to confounding factors.
AB - Objective: The aim of this study was to assess the responsiveness of two classes of bone resorption markers to the enhancement of osteoclastic activity induced by orchiectomy and to its inhibition by clodronate treatment in mature rats. Design: Bone mineral density (BMD) at femural metaphysis, femural diaphysis, lumbar vertebrae, and the urinary excretion of pyridinoline (Pyr), deoxypyridinoline (D-Pyr), galactosylhydroxylysine (GHyl) and glucosylgalactosylhydroxylysine (GGHyl) were monitored at regular intervals for 30 days prior to and for 60 days following orchiectomy in eleven rats, divided into two groups: five rats untreated and the other six treated with clodronate. Results: Prior to orchiectomy, a significant (P <0.01) decrease in BMD was observed only at the distal femural metaphysis. This decrease appeared to be associated with a time-dependent increase in the urinary excretion of all markers. Following orchiectomy, the BMD of the untreated group decreased significantly (P <0.01) at all bone sites. The bone loss was accompanied by a significant (P <0.01) increase in Pyr and D- Pyr concentrations in urine, whereas urinary GHyl and GGHyl did not change significantly. In the clodronate-treated group, the BMD of the three skeletal sites did not change significantly, while the urinary excretion of all urinary biochemical markers decreased significantly (P <0.001). Conclusion: This study showed that pyridinolines are able to monitor the bone response to orchiectomy and to clodronate treatment response in androgen-deficient mature male rat, whereas glycosides appear prone to confounding factors.
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U2 - 10.1530/eje.0.1380120
DO - 10.1530/eje.0.1380120
M3 - Article
C2 - 9461327
AN - SCOPUS:0031937542
VL - 138
SP - 120
EP - 127
JO - European Journal of Endocrinology
JF - European Journal of Endocrinology
SN - 0804-4643
IS - 1
ER -