Abstract
Only a minority of patients with chronic myeloid leukaemia (CML) benefit from allogeneic bone marrow transplantation (BMT), a potentially curative therapy, or from treatment with interferon alpha, which prolongs survival in cytogenetic responders. In Genoa a programme has been initiated in which CML patients are autografted with Ph-negative peripheral stem cells. To assess the pattern of marrow reconstitution, we studied the clonality of haemopoiesis in five females who engrafted and were Philadelphia chromosome negative. This was performed by evaluating the methylation patterns of the X-linked hypervariable DXS255 locus with the probe M27β. An four analysable women showed polyclonal methylation patterns in both granulocytes and T lymphocytes, suggesting that marrow reconstitution occurred from nornal residual stem cells.
| Original language | English |
|---|---|
| Pages (from-to) | 867-870 |
| Number of pages | 4 |
| Journal | British Journal of Haematology |
| Volume | 87 |
| Issue number | 4 |
| Publication status | Published - 1994 |
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Keywords
- Autograft
- CML
- Peripheral stem cell
- Ph-negative
- Polyclonal haemopoiesis
ASJC Scopus subject areas
- Hematology
Cite this
Restoration of normal polyclonal haemopoiesis in patients with chronic myeloid leukaemia autografted with Ph-negative peripheral stem cells. / Bergamaschi, G.; Podesta, M.; Frassoni, F.; Rosti, V.; Carella, A. M.; Saglio, G.; Cazzola, M.
In: British Journal of Haematology, Vol. 87, No. 4, 1994, p. 867-870.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Restoration of normal polyclonal haemopoiesis in patients with chronic myeloid leukaemia autografted with Ph-negative peripheral stem cells
AU - Bergamaschi, G.
AU - Podesta, M.
AU - Frassoni, F.
AU - Rosti, V.
AU - Carella, A. M.
AU - Saglio, G.
AU - Cazzola, M.
PY - 1994
Y1 - 1994
N2 - Only a minority of patients with chronic myeloid leukaemia (CML) benefit from allogeneic bone marrow transplantation (BMT), a potentially curative therapy, or from treatment with interferon alpha, which prolongs survival in cytogenetic responders. In Genoa a programme has been initiated in which CML patients are autografted with Ph-negative peripheral stem cells. To assess the pattern of marrow reconstitution, we studied the clonality of haemopoiesis in five females who engrafted and were Philadelphia chromosome negative. This was performed by evaluating the methylation patterns of the X-linked hypervariable DXS255 locus with the probe M27β. An four analysable women showed polyclonal methylation patterns in both granulocytes and T lymphocytes, suggesting that marrow reconstitution occurred from nornal residual stem cells.
AB - Only a minority of patients with chronic myeloid leukaemia (CML) benefit from allogeneic bone marrow transplantation (BMT), a potentially curative therapy, or from treatment with interferon alpha, which prolongs survival in cytogenetic responders. In Genoa a programme has been initiated in which CML patients are autografted with Ph-negative peripheral stem cells. To assess the pattern of marrow reconstitution, we studied the clonality of haemopoiesis in five females who engrafted and were Philadelphia chromosome negative. This was performed by evaluating the methylation patterns of the X-linked hypervariable DXS255 locus with the probe M27β. An four analysable women showed polyclonal methylation patterns in both granulocytes and T lymphocytes, suggesting that marrow reconstitution occurred from nornal residual stem cells.
KW - Autograft
KW - CML
KW - Peripheral stem cell
KW - Ph-negative
KW - Polyclonal haemopoiesis
UR - http://www.scopus.com/inward/record.url?scp=0028106392&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028106392&partnerID=8YFLogxK
M3 - Article
VL - 87
SP - 867
EP - 870
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 4
ER -