Restoration of normal polyclonal haemopoiesis in patients with chronic myeloid leukaemia autografted with Ph-negative peripheral stem cells

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Abstract

Only a minority of patients with chronic myeloid leukaemia (CML) benefit from allogeneic bone marrow transplantation (BMT), a potentially curative therapy, or from treatment with interferon alpha, which prolongs survival in cytogenetic responders. In Genoa a programme has been initiated in which CML patients are autografted with Ph-negative peripheral stem cells. To assess the pattern of marrow reconstitution, we studied the clonality of haemopoiesis in five females who engrafted and were Philadelphia chromosome negative. This was performed by evaluating the methylation patterns of the X-linked hypervariable DXS255 locus with the probe M27β. An four analysable women showed polyclonal methylation patterns in both granulocytes and T lymphocytes, suggesting that marrow reconstitution occurred from nornal residual stem cells.

Original languageEnglish
Pages (from-to)867-870
Number of pages4
JournalBritish Journal of Haematology
Volume87
Issue number4
Publication statusPublished - 1994

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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Methylation
Bone Marrow
Philadelphia Chromosome
Homologous Transplantation
Bone Marrow Transplantation
Granulocytes
Interferon-alpha
Cytogenetics
Stem Cells
T-Lymphocytes
Survival
Therapeutics
Peripheral Blood Stem Cells

Keywords

  • Autograft
  • CML
  • Peripheral stem cell
  • Ph-negative
  • Polyclonal haemopoiesis

ASJC Scopus subject areas

  • Hematology

Cite this

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abstract = "Only a minority of patients with chronic myeloid leukaemia (CML) benefit from allogeneic bone marrow transplantation (BMT), a potentially curative therapy, or from treatment with interferon alpha, which prolongs survival in cytogenetic responders. In Genoa a programme has been initiated in which CML patients are autografted with Ph-negative peripheral stem cells. To assess the pattern of marrow reconstitution, we studied the clonality of haemopoiesis in five females who engrafted and were Philadelphia chromosome negative. This was performed by evaluating the methylation patterns of the X-linked hypervariable DXS255 locus with the probe M27β. An four analysable women showed polyclonal methylation patterns in both granulocytes and T lymphocytes, suggesting that marrow reconstitution occurred from nornal residual stem cells.",
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T1 - Restoration of normal polyclonal haemopoiesis in patients with chronic myeloid leukaemia autografted with Ph-negative peripheral stem cells

AU - Bergamaschi, G.

AU - Podesta, M.

AU - Frassoni, F.

AU - Rosti, V.

AU - Carella, A. M.

AU - Saglio, G.

AU - Cazzola, M.

PY - 1994

Y1 - 1994

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AB - Only a minority of patients with chronic myeloid leukaemia (CML) benefit from allogeneic bone marrow transplantation (BMT), a potentially curative therapy, or from treatment with interferon alpha, which prolongs survival in cytogenetic responders. In Genoa a programme has been initiated in which CML patients are autografted with Ph-negative peripheral stem cells. To assess the pattern of marrow reconstitution, we studied the clonality of haemopoiesis in five females who engrafted and were Philadelphia chromosome negative. This was performed by evaluating the methylation patterns of the X-linked hypervariable DXS255 locus with the probe M27β. An four analysable women showed polyclonal methylation patterns in both granulocytes and T lymphocytes, suggesting that marrow reconstitution occurred from nornal residual stem cells.

KW - Autograft

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KW - Peripheral stem cell

KW - Ph-negative

KW - Polyclonal haemopoiesis

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