Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2

F. De Paola; R. Ridolfi; A. Riccobon; E. Flamini; F. Barzanti; A. M. Granato; G. L. Mordenti; L. Medri; P. Vitali; D. Amadori

doi:10.1038/sj.bjc.6600679

Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2

F. De Paola, R. Ridolfi, A. Riccobon, E. Flamini, F. Barzanti, A. M. Granato, G. L. Mordenti, L. Medri, P. Vitali, D. Amadori

Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori

Research output: Contribution to journal › Article

Abstract

We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) ε chain, p56^lck, Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR ε chain and p56^lck expression of TIL rose to median values of approximately 80 and 30% positive cells, respectively (P

Original language	English
Pages (from-to)	320-326
Number of pages	7
Journal	British Journal of Cancer
Volume	88
Issue number	2
DOIs	https://doi.org/10.1038/sj.bjc.6600679
Publication status	Published - Jan 27 2003

Keywords

IL-2
TIL
Tumour immunosuppression

ASJC Scopus subject areas

Cancer Research
Oncology

Access to Document

10.1038/sj.bjc.6600679

Fingerprint Dive into the research topics of 'Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2'. Together they form a unique fingerprint.

Cite this

De Paola, F., Ridolfi, R., Riccobon, A., Flamini, E., Barzanti, F., Granato, A. M., Mordenti, G. L., Medri, L., Vitali, P., & Amadori, D. (2003). Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2. British Journal of Cancer, 88(2), 320-326. https://doi.org/10.1038/sj.bjc.6600679

Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2. / De Paola, F.; Ridolfi, R.; Riccobon, A.; Flamini, E.; Barzanti, F.; Granato, A. M.; Mordenti, G. L.; Medri, L.; Vitali, P.; Amadori, D.

In: British Journal of Cancer, Vol. 88, No. 2, 27.01.2003, p. 320-326.

Research output: Contribution to journal › Article

De Paola, F, Ridolfi, R, Riccobon, A, Flamini, E, Barzanti, F, Granato, AM, Mordenti, GL, Medri, L, Vitali, P & Amadori, D 2003, 'Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2', British Journal of Cancer, vol. 88, no. 2, pp. 320-326. https://doi.org/10.1038/sj.bjc.6600679

@article{5271c41067e04386bbc376a4ba177b54,

title = "Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2",

abstract = "We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) ε chain, p56lck, Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR ε chain and p56lck expression of TIL rose to median values of approximately 80 and 30% positive cells, respectively (P",

keywords = "IL-2, TIL, Tumour immunosuppression",

author = "{De Paola}, F. and R. Ridolfi and A. Riccobon and E. Flamini and F. Barzanti and Granato, {A. M.} and Mordenti, {G. L.} and L. Medri and P. Vitali and D. Amadori",

year = "2003",

month = jan,

day = "27",

doi = "10.1038/sj.bjc.6600679",

language = "English",

volume = "88",

pages = "320--326",

journal = "British Journal of Cancer",

issn = "0007-0920",

publisher = "Nature Publishing Group",

number = "2",

}

TY - JOUR

T1 - Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2

AU - De Paola, F.

AU - Ridolfi, R.

AU - Riccobon, A.

AU - Flamini, E.

AU - Barzanti, F.

AU - Granato, A. M.

AU - Mordenti, G. L.

AU - Medri, L.

AU - Vitali, P.

AU - Amadori, D.

PY - 2003/1/27

Y1 - 2003/1/27

N2 - We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) ε chain, p56lck, Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR ε chain and p56lck expression of TIL rose to median values of approximately 80 and 30% positive cells, respectively (P

AB - We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) ε chain, p56lck, Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR ε chain and p56lck expression of TIL rose to median values of approximately 80 and 30% positive cells, respectively (P

KW - IL-2

KW - TIL

KW - Tumour immunosuppression

UR - http://www.scopus.com/inward/record.url?scp=0037468099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037468099&partnerID=8YFLogxK

U2 - 10.1038/sj.bjc.6600679

DO - 10.1038/sj.bjc.6600679

M3 - Article

C2 - 12610520

AN - SCOPUS:0037468099

VL - 88

SP - 320

EP - 326

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 2

ER -