Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2

F. De Paola, R. Ridolfi, A. Riccobon, E. Flamini, F. Barzanti, A. M. Granato, G. L. Mordenti, L. Medri, P. Vitali, D. Amadori

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) ε chain, p56lck, Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR ε chain and p56lck expression of TIL rose to median values of approximately 80 and 30% positive cells, respectively (P

Original languageEnglish
Pages (from-to)320-326
Number of pages7
JournalBritish Journal of Cancer
Volume88
Issue number2
DOIs
Publication statusPublished - Jan 27 2003

Fingerprint

Tumor-Infiltrating Lymphocytes
Interleukin-2
Colorectal Neoplasms
Melanoma
Fas Ligand Protein
T-Cell Antigen Receptor
T-Lymphocytes
Signal Transduction
Apoptosis
Neoplasm Metastasis
Neoplasms
Therapeutics

Keywords

  • IL-2
  • TIL
  • Tumour immunosuppression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2. / De Paola, F.; Ridolfi, R.; Riccobon, A.; Flamini, E.; Barzanti, F.; Granato, A. M.; Mordenti, G. L.; Medri, L.; Vitali, P.; Amadori, D.

In: British Journal of Cancer, Vol. 88, No. 2, 27.01.2003, p. 320-326.

Research output: Contribution to journalArticle

De Paola, F, Ridolfi, R, Riccobon, A, Flamini, E, Barzanti, F, Granato, AM, Mordenti, GL, Medri, L, Vitali, P & Amadori, D 2003, 'Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2', British Journal of Cancer, vol. 88, no. 2, pp. 320-326. https://doi.org/10.1038/sj.bjc.6600679
De Paola, F. ; Ridolfi, R. ; Riccobon, A. ; Flamini, E. ; Barzanti, F. ; Granato, A. M. ; Mordenti, G. L. ; Medri, L. ; Vitali, P. ; Amadori, D. / Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2. In: British Journal of Cancer. 2003 ; Vol. 88, No. 2. pp. 320-326.
@article{5271c41067e04386bbc376a4ba177b54,
title = "Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2",
abstract = "We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) ε chain, p56lck, Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR ε chain and p56lck expression of TIL rose to median values of approximately 80 and 30{\%} positive cells, respectively (P",
keywords = "IL-2, TIL, Tumour immunosuppression",
author = "{De Paola}, F. and R. Ridolfi and A. Riccobon and E. Flamini and F. Barzanti and Granato, {A. M.} and Mordenti, {G. L.} and L. Medri and P. Vitali and D. Amadori",
year = "2003",
month = "1",
day = "27",
doi = "10.1038/sj.bjc.6600679",
language = "English",
volume = "88",
pages = "320--326",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2

AU - De Paola, F.

AU - Ridolfi, R.

AU - Riccobon, A.

AU - Flamini, E.

AU - Barzanti, F.

AU - Granato, A. M.

AU - Mordenti, G. L.

AU - Medri, L.

AU - Vitali, P.

AU - Amadori, D.

PY - 2003/1/27

Y1 - 2003/1/27

N2 - We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) ε chain, p56lck, Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR ε chain and p56lck expression of TIL rose to median values of approximately 80 and 30% positive cells, respectively (P

AB - We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) ε chain, p56lck, Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR ε chain and p56lck expression of TIL rose to median values of approximately 80 and 30% positive cells, respectively (P

KW - IL-2

KW - TIL

KW - Tumour immunosuppression

UR - http://www.scopus.com/inward/record.url?scp=0037468099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037468099&partnerID=8YFLogxK

U2 - 10.1038/sj.bjc.6600679

DO - 10.1038/sj.bjc.6600679

M3 - Article

C2 - 12610520

AN - SCOPUS:0037468099

VL - 88

SP - 320

EP - 326

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 2

ER -