Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2

F. De Paola, R. Ridolfi, A. Riccobon, E. Flamini, F. Barzanti, A. M. Granato, G. L. Mordenti, L. Medri, P. Vitali, D. Amadori

Research output: Contribution to journalArticle

Abstract

We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) ε chain, p56lck, Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR ε chain and p56lck expression of TIL rose to median values of approximately 80 and 30% positive cells, respectively (P

Original languageEnglish
Pages (from-to)320-326
Number of pages7
JournalBritish Journal of Cancer
Volume88
Issue number2
DOIs
Publication statusPublished - Jan 27 2003

Keywords

  • IL-2
  • TIL
  • Tumour immunosuppression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    De Paola, F., Ridolfi, R., Riccobon, A., Flamini, E., Barzanti, F., Granato, A. M., Mordenti, G. L., Medri, L., Vitali, P., & Amadori, D. (2003). Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2. British Journal of Cancer, 88(2), 320-326. https://doi.org/10.1038/sj.bjc.6600679