Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2

F. De Paola; R. Ridolfi; A. Riccobon; E. Flamini; F. Barzanti; A. M. Granato; G. L. Mordenti; L. Medri; P. Vitali; D. Amadori

doi:10.1038/sj.bjc.6600679

Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2

F. De Paola, R. Ridolfi, A. Riccobon, E. Flamini, F. Barzanti, A. M. Granato, G. L. Mordenti, L. Medri, P. Vitali, D. Amadori

Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori

Research output: Contribution to journal › Article › peer-review

Abstract

We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) ε chain, p56^lck, Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR ε chain and p56^lck expression of TIL rose to median values of approximately 80 and 30% positive cells, respectively (P

Original language	English
Pages (from-to)	320-326
Number of pages	7
Journal	British Journal of Cancer
Volume	88
Issue number	2
DOIs	https://doi.org/10.1038/sj.bjc.6600679
Publication status	Published - Jan 27 2003

Keywords

IL-2
TIL
Tumour immunosuppression

ASJC Scopus subject areas

Cancer Research
Oncology

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10.1038/sj.bjc.6600679

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De Paola, F., Ridolfi, R., Riccobon, A., Flamini, E., Barzanti, F., Granato, A. M., Mordenti, G. L., Medri, L., Vitali, P., & Amadori, D. (2003). Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2. British Journal of Cancer, 88(2), 320-326. https://doi.org/10.1038/sj.bjc.6600679

De Paola, F, Ridolfi, R, Riccobon, A, Flamini, E, Barzanti, F, Granato, AM, Mordenti, GL, Medri, L, Vitali, P & Amadori, D 2003, 'Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2', British Journal of Cancer, vol. 88, no. 2, pp. 320-326. https://doi.org/10.1038/sj.bjc.6600679

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abstract = "We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) ε chain, p56lck, Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR ε chain and p56lck expression of TIL rose to median values of approximately 80 and 30% positive cells, respectively (P",

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AU - De Paola, F.

AU - Ridolfi, R.

AU - Riccobon, A.

AU - Flamini, E.

AU - Barzanti, F.

AU - Granato, A. M.

AU - Mordenti, G. L.

AU - Medri, L.

AU - Vitali, P.

AU - Amadori, D.

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AB - We investigated the effects of interleukin-2 (IL-2) exposure on T-cell signal transduction molecules and apoptosis markers in tumour-infiltrating lymphocytes (TIL) isolated from 20 melanoma and 16 colorectal carcinoma metastases and expanded in vitro for therapeutic reinfusion. Before IL-2 culture, TIL showed undetectable or very low levels of T-cell receptor (TCR) ε chain, p56lck, Fas ligand (FasL) and Bax expression, while Bcl-2 values were elevated. Cancer cells were characterised by low or absent Fas and Bcl-2 and high Bax expression. Notably, they also expressed FasL. After 41-48 days of IL-2 culture, TCR ε chain and p56lck expression of TIL rose to median values of approximately 80 and 30% positive cells, respectively (P

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Restored T-cell activation mechanisms in human tumour-infiltrating lymphocytes from melanomas and colorectal carcinomas after exposure to interleukin-2

Abstract

Keywords

ASJC Scopus subject areas

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