Abstract
Trans-resveratrol, a natural stilbene present in wine and grapes, has been studied mainly for its antiinflammatory and anticancer activities. In this study the activity of resveratrol on proliferative immunological parameters (differentiation, apoptosis, phagocytosis and intracellular killing) was studied using a U937 human promonocytic cell line in comparison with another polyphenol, quercetin. After incubation of the pathogen, Candida albicans, intracellular killing by macrophage-like cells was decreased by quercetin and resveratrol 10 μM but was enhanced by resveratrol 1 μM after 20 h of treatment. Phagocytosis rate, expressed as phagocytosis frequency, (i.e., percentage number of phagocytosing cells/total cells) at 20 h was highest with resveratrol 10 μM and was higher with quercetin 10 μM than with resveratrol 1 μM. The phagocytosis index exhibited the same trend. While both polyphenols demonstrated cytostatic activity on U937 growth, a prointraphagocytic effect for resveratrol 10 μM-treated cells at 10 min, resveratrol 1 μM-treated cells at 20 h and resveratrol 10 μM-treated cells at 48 h was observed. Morphological examination with optic microscopy demonstrated both apoptotic and differentiating cells, even after 10 min treatment. Resveratrol-induced apoptosis (following 4 h treatment) was confirmed by flow cytometry at concentrations as low as 1 μM and 100 nM in the assay for detection of membrane phosphatidylserine. Resveratrol- or quercetin-treated, but unstimulated cells, did not produce tumor necrosis factor-α protein. As phophatidylserine externalization triggers specific recognition by monocytes and macrophages, removal of intact apoptotic cells is important a) in cell population selection and differentiation for antiblastic therapy, and b) in preventing the release of toxic inflammatory substances such as reactive oxygen substances and proteolytic enzymes by dying cells. This observation suggests that wine polyphenols, at the same concentrations as those found in plasma after moderate wine consumption, are important cofactors in antiinfective, antiinflammatory and anticancer nonspecific immune reactions.
| Original language | English |
|---|---|
| Pages (from-to) | 93-104 |
| Number of pages | 12 |
| Journal | International Journal of Tissue Reactions |
| Volume | 21 |
| Issue number | 4 |
| Publication status | Published - 1999 |
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ASJC Scopus subject areas
- Physiology
- Cell Biology
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Resveratrol, a natural stilbene in grapes and wine, enhances intraphagocytosis in human promonocytes : A co-factor in antiinflammatory and anticancer chemopreventive activity. / Bertelli, Alberto A E; Ferrara, F.; Diana, G.; Fulgenzi, A.; Corsi, M.; Ponti, W.; Ferrero, M. E.; Bertelli, A.
In: International Journal of Tissue Reactions, Vol. 21, No. 4, 1999, p. 93-104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Resveratrol, a natural stilbene in grapes and wine, enhances intraphagocytosis in human promonocytes
T2 - A co-factor in antiinflammatory and anticancer chemopreventive activity
AU - Bertelli, Alberto A E
AU - Ferrara, F.
AU - Diana, G.
AU - Fulgenzi, A.
AU - Corsi, M.
AU - Ponti, W.
AU - Ferrero, M. E.
AU - Bertelli, A.
PY - 1999
Y1 - 1999
N2 - Trans-resveratrol, a natural stilbene present in wine and grapes, has been studied mainly for its antiinflammatory and anticancer activities. In this study the activity of resveratrol on proliferative immunological parameters (differentiation, apoptosis, phagocytosis and intracellular killing) was studied using a U937 human promonocytic cell line in comparison with another polyphenol, quercetin. After incubation of the pathogen, Candida albicans, intracellular killing by macrophage-like cells was decreased by quercetin and resveratrol 10 μM but was enhanced by resveratrol 1 μM after 20 h of treatment. Phagocytosis rate, expressed as phagocytosis frequency, (i.e., percentage number of phagocytosing cells/total cells) at 20 h was highest with resveratrol 10 μM and was higher with quercetin 10 μM than with resveratrol 1 μM. The phagocytosis index exhibited the same trend. While both polyphenols demonstrated cytostatic activity on U937 growth, a prointraphagocytic effect for resveratrol 10 μM-treated cells at 10 min, resveratrol 1 μM-treated cells at 20 h and resveratrol 10 μM-treated cells at 48 h was observed. Morphological examination with optic microscopy demonstrated both apoptotic and differentiating cells, even after 10 min treatment. Resveratrol-induced apoptosis (following 4 h treatment) was confirmed by flow cytometry at concentrations as low as 1 μM and 100 nM in the assay for detection of membrane phosphatidylserine. Resveratrol- or quercetin-treated, but unstimulated cells, did not produce tumor necrosis factor-α protein. As phophatidylserine externalization triggers specific recognition by monocytes and macrophages, removal of intact apoptotic cells is important a) in cell population selection and differentiation for antiblastic therapy, and b) in preventing the release of toxic inflammatory substances such as reactive oxygen substances and proteolytic enzymes by dying cells. This observation suggests that wine polyphenols, at the same concentrations as those found in plasma after moderate wine consumption, are important cofactors in antiinfective, antiinflammatory and anticancer nonspecific immune reactions.
AB - Trans-resveratrol, a natural stilbene present in wine and grapes, has been studied mainly for its antiinflammatory and anticancer activities. In this study the activity of resveratrol on proliferative immunological parameters (differentiation, apoptosis, phagocytosis and intracellular killing) was studied using a U937 human promonocytic cell line in comparison with another polyphenol, quercetin. After incubation of the pathogen, Candida albicans, intracellular killing by macrophage-like cells was decreased by quercetin and resveratrol 10 μM but was enhanced by resveratrol 1 μM after 20 h of treatment. Phagocytosis rate, expressed as phagocytosis frequency, (i.e., percentage number of phagocytosing cells/total cells) at 20 h was highest with resveratrol 10 μM and was higher with quercetin 10 μM than with resveratrol 1 μM. The phagocytosis index exhibited the same trend. While both polyphenols demonstrated cytostatic activity on U937 growth, a prointraphagocytic effect for resveratrol 10 μM-treated cells at 10 min, resveratrol 1 μM-treated cells at 20 h and resveratrol 10 μM-treated cells at 48 h was observed. Morphological examination with optic microscopy demonstrated both apoptotic and differentiating cells, even after 10 min treatment. Resveratrol-induced apoptosis (following 4 h treatment) was confirmed by flow cytometry at concentrations as low as 1 μM and 100 nM in the assay for detection of membrane phosphatidylserine. Resveratrol- or quercetin-treated, but unstimulated cells, did not produce tumor necrosis factor-α protein. As phophatidylserine externalization triggers specific recognition by monocytes and macrophages, removal of intact apoptotic cells is important a) in cell population selection and differentiation for antiblastic therapy, and b) in preventing the release of toxic inflammatory substances such as reactive oxygen substances and proteolytic enzymes by dying cells. This observation suggests that wine polyphenols, at the same concentrations as those found in plasma after moderate wine consumption, are important cofactors in antiinfective, antiinflammatory and anticancer nonspecific immune reactions.
UR - http://www.scopus.com/inward/record.url?scp=0033512798&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033512798&partnerID=8YFLogxK
M3 - Article
C2 - 10761539
AN - SCOPUS:0033512798
VL - 21
SP - 93
EP - 104
JO - International Journal of Tissue Reactions
JF - International Journal of Tissue Reactions
SN - 0250-0868
IS - 4
ER -