Resveratrol impairs the formation of MDA-MB-231 multicellular tumor spheroids concomitant with ceramide accumulation

Ersilia Dolfini, Leda Roncoroni, Elena Dogliotti, Giusy Sala, Eugenio Erba, Nicoletta Sacchi, Riccardo Ghidoni

Research output: Contribution to journalArticle

Abstract

Resveratrol exerts a drastic growth inhibitory effect on human breast cancer MDA-MB-231 cells grown both in vitro and in vivo. Here we show that resveratrol affects the aggregation properties of MDA-MB-231 cells into multicellular tumor spheroids (MCTSs), in association with induction of de novo synthesis of ceramide. After 9 days of 3D growth in the presence of resveratrol (64 μM), MDA-MB-231 cells formed significantly smaller MCTSs. Further, cells from these aggregates failed to form colonies. Addition of resveratrol (64 μM) to preformed MDA-MB-231 MCTSs caused no significant size change, consistent with lack of ceramide induction. Only some apoptotic blebs were found on the MCTSs surface. Altogether these findings indicate that resveratrol might be effective for prevention of breast cancer cell growth.

Original languageEnglish
Pages (from-to)143-147
Number of pages5
JournalCancer Letters
Volume249
Issue number2
DOIs
Publication statusPublished - May 8 2007

Keywords

  • Breast cancer cells
  • Ceramide
  • Resveratrol

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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