Resveratrol inhibits the epidermal growth factor-induced epithelial mesenchymal transition in MCF-7 cells

Daniele Vergara, Concetta Maria Valente, Andrea Tinelli, Carlo Siciliano, Vito Lorusso, Raffaele Acierno, Giovanna Giovinazzo, Angelo Santino, Carlo Storelli, Michele Maffia

Research output: Contribution to journalArticle

Abstract

Carcinoma progression is associated with the loss of epithelial features, and the acquisition of a mesenchymal phenotype by tumour cells. Herein we show that exposure of MCF-7 cells to epidermal growth factor (EGF) resulted in morphological alterations characteristic of epithelial-to-mesenchymal transition (EMT). EGF treatment resulted in increased motility along with an up-regulation of transcription factors Slug, Zeb1, Zeb2, and mesenchymal markers Vimentin and N-cadherin.Treatment of MCF-7 cells with a combined stimulation of EGF and resveratrol, a naturally occurring stilbene with antitumor properties, failed to alter cell morphology, motility and overexpression of EMT markers induced by EGF. Using specific chemical inhibitors, we demonstrated that EGF-induced EMT is mediated by extracellular signal-regulated kinase 1/2 (ERK 1/2) signalling pathway and that resveratrol is able to repress EGF-induced ERK activation.In summary, these data provide new evidence of the inhibitory effect of resveratrol on EGF-induced EMT cell transformation.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalCancer Letters
Volume310
Issue number1
DOIs
Publication statusPublished - Nov 1 2011

Keywords

  • EGF
  • Epithelial-mesenchymal transition
  • MCF-7
  • Resveratrol

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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  • Cite this

    Vergara, D., Valente, C. M., Tinelli, A., Siciliano, C., Lorusso, V., Acierno, R., Giovinazzo, G., Santino, A., Storelli, C., & Maffia, M. (2011). Resveratrol inhibits the epidermal growth factor-induced epithelial mesenchymal transition in MCF-7 cells. Cancer Letters, 310(1), 1-8. https://doi.org/10.1016/j.canlet.2011.04.009