Resveratrol inhibits the epidermal growth factor-induced epithelial mesenchymal transition in MCF-7 cells

Daniele Vergara, Concetta Maria Valente, Andrea Tinelli, Carlo Siciliano, Vito Lorusso, Raffaele Acierno, Giovanna Giovinazzo, Angelo Santino, Carlo Storelli, Michele Maffia

Research output: Contribution to journalArticle

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Abstract

Carcinoma progression is associated with the loss of epithelial features, and the acquisition of a mesenchymal phenotype by tumour cells. Herein we show that exposure of MCF-7 cells to epidermal growth factor (EGF) resulted in morphological alterations characteristic of epithelial-to-mesenchymal transition (EMT). EGF treatment resulted in increased motility along with an up-regulation of transcription factors Slug, Zeb1, Zeb2, and mesenchymal markers Vimentin and N-cadherin.Treatment of MCF-7 cells with a combined stimulation of EGF and resveratrol, a naturally occurring stilbene with antitumor properties, failed to alter cell morphology, motility and overexpression of EMT markers induced by EGF. Using specific chemical inhibitors, we demonstrated that EGF-induced EMT is mediated by extracellular signal-regulated kinase 1/2 (ERK 1/2) signalling pathway and that resveratrol is able to repress EGF-induced ERK activation.In summary, these data provide new evidence of the inhibitory effect of resveratrol on EGF-induced EMT cell transformation.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalCancer Letters
Volume310
Issue number1
DOIs
Publication statusPublished - Nov 1 2011

Fingerprint

Epithelial-Mesenchymal Transition
MCF-7 Cells
Epidermal Growth Factor
Stilbenes
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Vimentin
Cadherins
resveratrol
Cell Movement
Up-Regulation
Carcinoma
Phenotype
Neoplasms

Keywords

  • EGF
  • Epithelial-mesenchymal transition
  • MCF-7
  • Resveratrol

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Resveratrol inhibits the epidermal growth factor-induced epithelial mesenchymal transition in MCF-7 cells. / Vergara, Daniele; Valente, Concetta Maria; Tinelli, Andrea; Siciliano, Carlo; Lorusso, Vito; Acierno, Raffaele; Giovinazzo, Giovanna; Santino, Angelo; Storelli, Carlo; Maffia, Michele.

In: Cancer Letters, Vol. 310, No. 1, 01.11.2011, p. 1-8.

Research output: Contribution to journalArticle

Vergara, D, Valente, CM, Tinelli, A, Siciliano, C, Lorusso, V, Acierno, R, Giovinazzo, G, Santino, A, Storelli, C & Maffia, M 2011, 'Resveratrol inhibits the epidermal growth factor-induced epithelial mesenchymal transition in MCF-7 cells', Cancer Letters, vol. 310, no. 1, pp. 1-8. https://doi.org/10.1016/j.canlet.2011.04.009
Vergara, Daniele ; Valente, Concetta Maria ; Tinelli, Andrea ; Siciliano, Carlo ; Lorusso, Vito ; Acierno, Raffaele ; Giovinazzo, Giovanna ; Santino, Angelo ; Storelli, Carlo ; Maffia, Michele. / Resveratrol inhibits the epidermal growth factor-induced epithelial mesenchymal transition in MCF-7 cells. In: Cancer Letters. 2011 ; Vol. 310, No. 1. pp. 1-8.
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