Resveratrol prevents apoptosis in K562 cells by inhibiting lipoxygenase and cyclooxygenase activity

Mauro Maccarrone, Tatiana Lorenzon, Pietro Guerrieri, Alessandro Finazzi Agrò

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

The natural polyphenolic compound resveratrol (trans-3,4',5- trihydroxystilbene) is shown to prevent apoptosis (programmed cell death) induced in human erythroleukemia K562 cells by hydrogen peroxide and other unrelated stimuli. Resveratrol reversed the elevation of leukotriene B4 (from 6.40 ± 0.65 to 2.92 ± 0.30 pmol·mg protein-1) and prostaglandin E2 (from 11.46 ± 1.15 to 8.02 ± 0.80 nmol·mg protein-1), induced by H2O2 challenge in K562 cells. The reduction of leukotriene B4 and prostaglandin E2 correlated with the inhibition of the 5-lipoxygenase activity, and the cyclooxygenase and peroxidase activity of prostaglandin H synthase, respectively. Resveratrol also blocked lipoperoxidation induced by hydrogen peroxide in K562 cell membranes. Resveratrol was found to act as a competitive inhibitor of purified 5-lipoxygenase and 15-lipoxygenase and prostaglandin H synthase, with inhibition constants of 4.5 ± 0.5 μM (5- lipoxygenase), 40 ± 5.0 μM (15-lipoxygenase), 35 ± 4.0 μM (cyclooxygenase activity of prostaglandin H synthase) and 30 ± 3.0 μM (peroxidase activity of prostaglandin H synthase). Altogether, the results reported here suggest that the anti-apoptotic activity of resveratrol depends on the direct inhibition of the main arachidonate-metabolizing enzymes.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalEuropean Journal of Biochemistry
Volume265
Issue number1
DOIs
Publication statusPublished - Oct 1 1999

Fingerprint

Lipoxygenase
K562 Cells
Prostaglandin-Endoperoxide Synthases
Apoptosis
Arachidonate 5-Lipoxygenase
Arachidonate 15-Lipoxygenase
Leukotriene B4
Dinoprostone
Hydrogen Peroxide
Peroxidase
Polyphenolic compounds
Lipoxygenase Inhibitors
Leukemia, Erythroblastic, Acute
Cell death
Cell membranes
resveratrol
Proteins
Cell Death
Cell Membrane
Enzymes

Keywords

  • Apoptosis
  • Cyclooxygenase
  • Lipoxygenase
  • Oxidative stress
  • Resveratrol

ASJC Scopus subject areas

  • Biochemistry

Cite this

Resveratrol prevents apoptosis in K562 cells by inhibiting lipoxygenase and cyclooxygenase activity. / Maccarrone, Mauro; Lorenzon, Tatiana; Guerrieri, Pietro; Finazzi Agrò, Alessandro.

In: European Journal of Biochemistry, Vol. 265, No. 1, 01.10.1999, p. 27-34.

Research output: Contribution to journalArticle

Maccarrone, Mauro ; Lorenzon, Tatiana ; Guerrieri, Pietro ; Finazzi Agrò, Alessandro. / Resveratrol prevents apoptosis in K562 cells by inhibiting lipoxygenase and cyclooxygenase activity. In: European Journal of Biochemistry. 1999 ; Vol. 265, No. 1. pp. 27-34.
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