TY - JOUR
T1 - Retaining the long-survive capacity of Circulating Tumor Cells (CTCs) followed by xeno-transplantation
T2 - Not only from metastatic cancer of the breast but also of prostate cancer patients
AU - Rossi, Elisabetta
AU - Rugge, Massimo
AU - Facchinetti, Antonella
AU - Pizzi, Marco
AU - Nardo, Giorgia
AU - Barbieri, Vito
AU - Manicone, Mariangela
AU - Faveri, Stefania De
AU - Scaini, Maria Chiara
AU - Basso, Umberto
AU - Amadori, Alberto
AU - Zamarchi, Rita
PY - 2014
Y1 - 2014
N2 - We investigated whether Circulating Tumor Cells (CTCs) isolated from epithelial tumors could survive and grow in xenotransplants. To this purpose, EpCAM-positive CTCs were enriched by CellSearch platform the only FDA-cleared automated platform that quantifies tumor burden in peripheral blood and provides clinical evidence of predictive and prognostic value. The CTCs were isolated from metastatic prostate (n=6) and breast (n=2) cancer patients. The xenograft assay was developed in 8-week-old NOD/SCID mice that were subcutaneously injected with increasing amounts of CTCs (ranging from 50 to 3000). Human CTCs were found in 8 out of 8 murine peripheral blood (muPB) and in 6 out of 8 murine bone marrow (muBM) samples, after a median follow-up of 10.3 months. Six out of 8 spleens were positive for human cytokeratin. Our assay showed higher successful rate than those previously reported in breast cancer and hepatocellular carcinoma. The role of EpCAM during carcinogenesis is controversial. The identification of human CTCs in muPB, muBM and spleen demonstrates that the EpCAM-positive fraction of CTCs retains the migratory capacity. This is the first experimental evidence that as few as 50 EpCAM-positive prostate cancer CTCs putatively contain metastasisinitiating- cells (MIC).
AB - We investigated whether Circulating Tumor Cells (CTCs) isolated from epithelial tumors could survive and grow in xenotransplants. To this purpose, EpCAM-positive CTCs were enriched by CellSearch platform the only FDA-cleared automated platform that quantifies tumor burden in peripheral blood and provides clinical evidence of predictive and prognostic value. The CTCs were isolated from metastatic prostate (n=6) and breast (n=2) cancer patients. The xenograft assay was developed in 8-week-old NOD/SCID mice that were subcutaneously injected with increasing amounts of CTCs (ranging from 50 to 3000). Human CTCs were found in 8 out of 8 murine peripheral blood (muPB) and in 6 out of 8 murine bone marrow (muBM) samples, after a median follow-up of 10.3 months. Six out of 8 spleens were positive for human cytokeratin. Our assay showed higher successful rate than those previously reported in breast cancer and hepatocellular carcinoma. The role of EpCAM during carcinogenesis is controversial. The identification of human CTCs in muPB, muBM and spleen demonstrates that the EpCAM-positive fraction of CTCs retains the migratory capacity. This is the first experimental evidence that as few as 50 EpCAM-positive prostate cancer CTCs putatively contain metastasisinitiating- cells (MIC).
KW - Breast cancer
KW - Circulating Tumor Cells
KW - EpCAM
KW - Prostate cancer
KW - Xenograft assay
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U2 - 10.18632/oncoscience.8
DO - 10.18632/oncoscience.8
M3 - Article
AN - SCOPUS:84931332727
VL - 1
SP - 49
EP - 56
JO - Oncoscience
JF - Oncoscience
SN - 2331-4737
IS - 1
ER -