Abstract
We have recently selected MAbs specific for different molecular forms of the TCR gamma/delta (expressed by distinct cell subsets), able to activate TCR gamma/delta+ cells. Two of these reagents (Gl and A13) were used for the construction of bispecific MAbs in conjunction with a MAb (Mov 19) directed to ovarian carcinoma cells, using the hybrid hybridoma technique. The Gl‐derived bispecific MAb GM33.9 efficiently induced lysis of Mov19 ovarian carcinoma cell lines (IGROV1 and SW626) by Gl+ clones in a 4‐hr 51Cr‐release assay. On the other hand, it was ineffective when Mov19− target cells were used. Comparable results were obtained with the A13‐derived AM18.4 bispecific MAb when A13+ clones were used as effector cells. Bispecific MAbs were also able to induce secretion of IL‐2 and TNF‐alpha by TCR gamma/delta+ clones when Mov19+ target cells were present.
| Original language | English |
|---|---|
| Pages (from-to) | 53-55 |
| Number of pages | 3 |
| Journal | International Journal of Cancer |
| Volume | 44 |
| Issue number | 1 S |
| DOIs | |
| Publication status | Published - 1989 |
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ASJC Scopus subject areas
- Medicine(all)
- Oncology
- Cancer Research
Cite this
Retargeting of T‐cell‐receptor gamma/delta+ lymphocytes against tumor cells by bispecific monoclonal antibodies. Induction of cytolytic activity and lymphokine production. / Ferrini, Silvano; Prigione, Ignazia; Mammoliti, Serafina; Colnaghi, Maria Ines; Menard, Sylvie; Moretta, Alessandro; Moretta, Lorenzo.
In: International Journal of Cancer, Vol. 44, No. 1 S, 1989, p. 53-55.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Retargeting of T‐cell‐receptor gamma/delta+ lymphocytes against tumor cells by bispecific monoclonal antibodies. Induction of cytolytic activity and lymphokine production
AU - Ferrini, Silvano
AU - Prigione, Ignazia
AU - Mammoliti, Serafina
AU - Colnaghi, Maria Ines
AU - Menard, Sylvie
AU - Moretta, Alessandro
AU - Moretta, Lorenzo
PY - 1989
Y1 - 1989
N2 - We have recently selected MAbs specific for different molecular forms of the TCR gamma/delta (expressed by distinct cell subsets), able to activate TCR gamma/delta+ cells. Two of these reagents (Gl and A13) were used for the construction of bispecific MAbs in conjunction with a MAb (Mov 19) directed to ovarian carcinoma cells, using the hybrid hybridoma technique. The Gl‐derived bispecific MAb GM33.9 efficiently induced lysis of Mov19 ovarian carcinoma cell lines (IGROV1 and SW626) by Gl+ clones in a 4‐hr 51Cr‐release assay. On the other hand, it was ineffective when Mov19− target cells were used. Comparable results were obtained with the A13‐derived AM18.4 bispecific MAb when A13+ clones were used as effector cells. Bispecific MAbs were also able to induce secretion of IL‐2 and TNF‐alpha by TCR gamma/delta+ clones when Mov19+ target cells were present.
AB - We have recently selected MAbs specific for different molecular forms of the TCR gamma/delta (expressed by distinct cell subsets), able to activate TCR gamma/delta+ cells. Two of these reagents (Gl and A13) were used for the construction of bispecific MAbs in conjunction with a MAb (Mov 19) directed to ovarian carcinoma cells, using the hybrid hybridoma technique. The Gl‐derived bispecific MAb GM33.9 efficiently induced lysis of Mov19 ovarian carcinoma cell lines (IGROV1 and SW626) by Gl+ clones in a 4‐hr 51Cr‐release assay. On the other hand, it was ineffective when Mov19− target cells were used. Comparable results were obtained with the A13‐derived AM18.4 bispecific MAb when A13+ clones were used as effector cells. Bispecific MAbs were also able to induce secretion of IL‐2 and TNF‐alpha by TCR gamma/delta+ clones when Mov19+ target cells were present.
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U2 - 10.1002/ijc.2910440714
DO - 10.1002/ijc.2910440714
M3 - Article
VL - 44
SP - 53
EP - 55
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 1 S
ER -