Reticulon protein-1C is a key component of MAMs

Valentina Reali, Bisan Mehdawy, Roberta Nardacci, Giuseppe Filomeni, Anna Risuglia, Federica Rossin, Manuela Antonioli, Claudia Marsella, Gian Maria Fimia, Mauro Piacentini, Federica Di Sano

Research output: Contribution to journalArticle

Abstract

The endoplasmic reticulum (ER) is a key organelle fundamental for the maintenance of cellular homeostasis and the determination of cell fate under stress conditions. Reticulon-1C (RTN-1C) is a member of the reticulon family proteins localized primarily on the ER membrane and known to regulate ER structure and function. Several cellular processes depend on the structural and functional crosstalk between different organelles, particularly on the endoplasmic reticulum and mitochondria. These dynamic contacts, called mitochondria-associated ER membranes (MAMs), are essential for the maintenance of mitochondrial structure and participate in lipid and calcium exchanges between the two organelles.In this study we investigated the impact of RTN-1C modulation on mitochondrial dynamics. We demonstrate that RTN-1C controls mitochondrial structure and function affecting intracellular Ca2+ homeostasis and lipid exchange between ER and mitochondria. We propose that these events depend on RTN-1C involvement in the regulation of ER-mitochondria cross-talk and define a role for RTN-1C in maintaining the function of contacts between the two organelles.

Original languageEnglish
Pages (from-to)733-745
Number of pages13
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1853
Issue number3
DOIs
Publication statusPublished - Mar 1 2015

    Fingerprint

Keywords

  • Endoplasmic reticulum
  • MAM
  • Mitochondria
  • RTN-1C

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this