Retinoic acid-induced modulation of rat liver transglutaminase and total polyamines in vivo

M. Piacentini, L. Fesus, C. Sartori, M. P. Ceru'

Research output: Contribution to journalArticle

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Abstract

The effect of a single intraperitoneal injection of retinoic acid on liver transglutaminase (EC 2.3.2.13) activity and total putrescine, spermidine and spermine was studied. The results demonstrate that: (1) transglutaminase activity is increased over control values as early as 4-6 h after treatment, reaching a maximum (2-fold increase) at 12 h and returning to control values at 36 h; (2) the retinoic acid-induced form of enzyme is the soluble tissue transglutaminase; (3) actinomycin D treatment does not completely inhibit the early (6 h) increase of activity, while suppressing that at 12 h; (4) the immunoassay of the soluble transglutaminase shows that, 6 h after treatment, there is no increase in the protein, whereas at 12 and 24 h a significant increase is observed; (5) putrescine, but not spermidine and spermine, increases (5-7-fold) 6 and 18 h after the retinoic acid treatment. The possibility also that the expression of soluble transglutaminase is modulated in vivo by retinoic acid and the relationship to polyamine levels are discussed.

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalBiochemical Journal
Volume253
Issue number1
Publication statusPublished - 1988

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Transglutaminases
Polyamines
Tretinoin
Liver
Rats
Modulation
Putrescine
Spermidine
Spermine
Dactinomycin
Therapeutics
Intraperitoneal Injections
Immunoassay
Enzymes
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Retinoic acid-induced modulation of rat liver transglutaminase and total polyamines in vivo. / Piacentini, M.; Fesus, L.; Sartori, C.; Ceru', M. P.

In: Biochemical Journal, Vol. 253, No. 1, 1988, p. 33-38.

Research output: Contribution to journalArticle

Piacentini, M. ; Fesus, L. ; Sartori, C. ; Ceru', M. P. / Retinoic acid-induced modulation of rat liver transglutaminase and total polyamines in vivo. In: Biochemical Journal. 1988 ; Vol. 253, No. 1. pp. 33-38.
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