@article{5f104bdc0bc74e6681cac34fafc39573,
title = "Retinoic Acid Induces Embryonic Stem Cells (ESCs) Transition to 2 Cell-Like State Through a Coordinated Expression of Dux and Duxbl1",
abstract = "Embryonic stem cells (ESCs) are derived from inner cell mass (ICM) of the blastocyst. In serum/LIF culture condition, they show variable expression of pluripotency genes that mark cell fluctuation between pluripotency and differentiation metastate. The ESCs subpopulation marked by zygotic genome activation gene (ZGA) signature, including Zscan4, retains a wider differentiation potency than epiblast-derived ESCs. We have recently shown that retinoic acid (RA) significantly enhances Zscan4 cell population. However, it remains unexplored how RA initiates the ESCs to 2-cell like reprogramming. Here we found that RA is decisive for ESCs to 2C-like cell transition, and reconstructed the gene network surrounding Zscan4. We revealed that RA regulates 2C-like population co-activating Dux and Duxbl1. We provided novel evidence that RA dependent ESCs to 2C-like cell transition is regulated by Dux, and antagonized by Duxbl1. Our suggested mechanism could shed light on the role of RA on ESC reprogramming.",
keywords = "2-cell like, ESCs, metastate, pluripotency, retinoic acid",
author = "Daniela Tagliaferri and Pellegrino Mazzone and Noviello, {Teresa M.R.} and Martina Addeo and Tiziana Angrisano and {Del Vecchio}, Luigi and Feliciano Visconte and Vitalba Ruggieri and Sabino Russi and Antonella Caivano and Irene Cantone and {De Felice}, Mario and Michele Ceccarelli and Luigi Cerulo and Geppino Falco",
note = "Funding Information: We thank Dr. Maria Vivo for critical reading and revision of the manuscript, Dr. De Iaco for the kind gift of the Dux-ko ESCs and Dux-Ha plasmid, Prof. Maurizio Ventre, and Dr. Carlo Natale from University of Naples Federico II for providing the Supplementary Video. This work was supported by STAR Linea 1, 2014 (University of Naples Federico II) to GF and Ministero dell' Istruzione, dell' Universit? e della Ricerca [PRIN 2017 XJ38A4 to GF]. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2020 Tagliaferri, Mazzone, Noviello, Addeo, Angrisano, Del Vecchio, Visconte, Ruggieri, Russi, Caivano, Cantone, De Felice, Ceccarelli, Cerulo and Falco. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = jan,
day = "17",
doi = "10.3389/fcell.2019.00385",
language = "English",
volume = "7",
journal = "Frontiers in Cell and Developmental Biology",
issn = "2296-634X",
publisher = "Frontiers Media S. A.",
}