Retinoic acid induces liver/bone/kidney-type alkaline phosphatase gene expression in F9 teratocarcinoma cells

M. Gianni', M. Studer, G. Carpani, M. Terao, E. Garattini

Research output: Contribution to journalArticle

Abstract

All-trans retinoic acid (RA) induces alkaline phosphatase (ALP) activity by 3-8-fold in murine F9 teratocarcinoma cells, in parallel with their differentiation towards primitive endoderm. The elevation of ALP activity is associated with increases in the amounts of liver/bone/kidney-type ALP protein and the respective transcript. These effects of RA are due to activation of ALP gene transcription rather than to an increase in the half-life of the mRNA. Induction of ALP mRNA does not require de novo protein synthesis, since it is not blocked by treatment with cycloheximide. Dibutyryl cyclic AMP, which is known to induce further differentiation of F9 cells from the primitive to the parietal endoderm, blocks the induction of ALP mRNA by RA.

Original languageEnglish
Pages (from-to)673-678
Number of pages6
JournalBiochemical Journal
Volume274
Issue number3
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Biochemistry

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