Retinoic acid induces stage-specific antero-posterior transformation of rostral central nervous system

Antonio Simeone, Virginia Avantaggiato, Maria Cristina Moroni, Fulvio Mavilio, Claudio Arra, Franco Cotelli, Vincenzo Nigro, Dario Acampora

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

We report a time-course analysis of the effect of retinoic acid (RA) on the development of the mouse central nervous system (CNS) from the beginning of gastrulation throughout induction and patterning of the neural tube. RA administration induces three different, stage-specific alterations of brain development, indicating perturbation of different morphogenetic steps during the establishment of a neural pattern. In particular, treatment at mid-late streak stage (7.2-7.4 days post coitum (d.p.c.)) results in early repression of Otx2 expression in the posterior neuroectoderm of the head fold and in the ventral mid line, including the prechordal plate and the rostralmost endoderm, followed by loss of forebrain morphological and molecular identities, as revealed by analysis of the expression of regionally-restricted brain genes (Otx2, Otx1, Emx2, Emx1 and Dlx1). In these embryos, reduction of the Otx2 expression domain correlates with hindbrain expansion marked by rostral extension of the Hoxb-1 expression domain. Our analysis indicates that RA interferes with the correct definition of both planar and vertical morphogenetic signals at specific developmental stages by affecting gene expression in the regions which are likely either to produce or to respond to these signals. We suggest that retinoids may contribute to early definition of head from trunk structures by selecting different sets of regulatory genes.

Original languageEnglish
Pages (from-to)83-98
Number of pages16
JournalMechanisms of Development
Volume51
Issue number1
DOIs
Publication statusPublished - 1995

Fingerprint

Tretinoin
Central Nervous System
Multifetal Pregnancy Reduction
Head
Neural Plate
Gastrulation
Rhombencephalon
Endoderm
Neural Tube
Retinoids
Brain
Regulator Genes
Prosencephalon
Gene Expression
Genes

Keywords

  • Forebrain
  • Gastrulation
  • Neural pattern
  • Otx2
  • Retinoic acid

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology
  • Developmental Neuroscience

Cite this

Retinoic acid induces stage-specific antero-posterior transformation of rostral central nervous system. / Simeone, Antonio; Avantaggiato, Virginia; Moroni, Maria Cristina; Mavilio, Fulvio; Arra, Claudio; Cotelli, Franco; Nigro, Vincenzo; Acampora, Dario.

In: Mechanisms of Development, Vol. 51, No. 1, 1995, p. 83-98.

Research output: Contribution to journalArticle

Simeone, Antonio ; Avantaggiato, Virginia ; Moroni, Maria Cristina ; Mavilio, Fulvio ; Arra, Claudio ; Cotelli, Franco ; Nigro, Vincenzo ; Acampora, Dario. / Retinoic acid induces stage-specific antero-posterior transformation of rostral central nervous system. In: Mechanisms of Development. 1995 ; Vol. 51, No. 1. pp. 83-98.
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