Retinoic acid inhibits phosphatidylinositol turnover only in RA-sensitive while not in RA-resistant human neuroblastoma cells

M. Lanciotti, P. Longone, P. Cornaglia-Ferraris, M. Ponzoni

Research output: Contribution to journalArticlepeer-review

Abstract

Phosphatidylinositol (PI) turnover has recently been implicated in the regulation of cell proliferation and transformation. We have investigated its role in differentiation using LAN-1 cells, a human neuroblastoma cell line which can be induced to differentiate along the neuronal pathway by retinoic acid (RA), and a derivated RA-resistant subline of it (LAN-1-res). We have found that treatment of LAN-1 cells with RA is followed by a rapid decrease of inositol phospholipid metabolism, using myo-[1,2-3H] inositol or [1,(3)-3H] glycerol. Analysis of labelled phosphatidylinositol metabolites from prelabelled LAN-1 cells indicated a rapid decrease of inositol (1,4,5)-trisphosphate and (1,2) diacylglycerol within 1 min. of induction of differentiation by RA, while no changes were observed in RA-treated LAN-1-res cells. These findings indicate that phosphoinositides-derived metabolites may be directly implicated in the induction processes of RA-triggered NB cell differentiation.

Original languageEnglish
Pages (from-to)284-289
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume161
Issue number1
DOIs
Publication statusPublished - May 30 1989

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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