Retinoic acid rapidly decreases phosphatidylinositol turnover during neuroblastoma cell differentiation

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Abstract

Phosphatidylinositol (PI) turnover has recently been implicated in the regulation of cell proliferation and transformation. We have investigated its role in differentiation using LAN-1 cells, a human neuroblastoma cell line that can be induced to differentiate along the neuronal pathway by retinoic acid (RA). We have found that treatment of LAN-1 cells with RA is followed by a rapid decrease of inositol phospholipid metabolism, using myo-[1,2-3H]inositol or [1(3)-3H]glycerol. No changes were observed in both [3H]inositol and [3H]glycerol uptake within 24 h of RA treatment. Decreased incorporation of the metabolic precursor into PI 4-monophosphate and PI 4,5-bisphosphate occurred within 1 h of RA treatment. No changes were seen in the specific radioactivity of the precursor pools up to 1 h of treatment with RA. Analysis of labeled PI metabolites from prelabeled cells indicated a rapid decrease of inositol 1,4,5-trisphosphate and 1,2-diacylglycerol content within 1 min of induction of LAN-1 cell differentiation. These findings constitute the earliest reported events in neuroblastoma cell differentiation.

Original languageEnglish
Pages (from-to)540-546
Number of pages7
JournalJournal of Neurochemistry
Volume54
Issue number2
DOIs
Publication statusPublished - 1990

Keywords

  • Neuroblastoma cell differentiation
  • Phosphatidylinositol turnover
  • Retinoic acid

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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