Retrieval of hemopoietic progenitors from buffy-coats filtered through a commercial white cell reduction filter

P. Rebulla, L. Sabbioneda, N. De Bernardi, L. Lazzari, L. Porretti, G. Puglisi, L. Lecchi, G. Sirchia

Research output: Contribution to journalArticle

Abstract

Purpose. We were interested in evaluating if filters used for white cell reduction of blood components can be used as a source of hemopoietic progenitors. Methods. We used for this study 50 mL buffy-coats (BC) from routine blood donations from healthy donors. We filtered the BC through polyurethane Imugard III-RC filters (Terumo, Tokyo, Japan), originally developed for white cell reduction of RBC. In 6 studies, which were performed within 24 hours of blood donation, we diluted the BC vol/vol with Plasmalyte-A (PL, Baxter, Deerfield, IL), primed the filter with PL, filtered the diluted BC by gravity at RT and rinsed the filter with 50 mL of PL. We then flushed vigorously the filler from outlet to inlet with 350 mL of PL and retrieved the cells in a transfer bag. We finally determined the percent recovery (final versus initial counts per unit) of mononuclear cells (MNC) by a Sismex 9000 hematology counter, the number of CD34+ cells by flow cytometry and of CFU-GM by 2-week culture in methylcellulose. Results. Pre-filtralion BC values/unit were: MNC 770±261×106; CD34+ cells 2.4±1.9×106; CFU-GM 91±73×103. Recoveries after elution were: MNC 93.5±20.7%; CD34+ cells 73.5±13.9%; CFU-GM 96.8±27.1%. Conclusions. A proportion of hemopoietic progenitors present in BC from routine donations can be collected after BC filtration through a commercial white cell reduction filter by filter elution with a crystalloid solution suitable for human use. Our preliminary observations suggest that commercial white cell reduction filters may be a useful tool for collection and retrieval of hemopoietic progenitors from blood components.

Original languageEnglish
Pages (from-to)239
Number of pages1
JournalInfusionstherapie und Transfusionsmedizin
Volume24
Issue number4
Publication statusPublished - 1997

Fingerprint

Granulocyte-Macrophage Progenitor Cells
cells
Blood Donors
blood
Methylcellulose
Polyurethanes
Tokyo
Gravitation
filling materials
Hematology
methylcellulose
polyurethanes
hematology
Flow Cytometry
Japan
Leukocytes
gravity
Cell Count
Tissue Donors
flow cytometry

ASJC Scopus subject areas

  • Hematology
  • Immunology
  • Food Science

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Retrieval of hemopoietic progenitors from buffy-coats filtered through a commercial white cell reduction filter. / Rebulla, P.; Sabbioneda, L.; De Bernardi, N.; Lazzari, L.; Porretti, L.; Puglisi, G.; Lecchi, L.; Sirchia, G.

In: Infusionstherapie und Transfusionsmedizin, Vol. 24, No. 4, 1997, p. 239.

Research output: Contribution to journalArticle

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abstract = "Purpose. We were interested in evaluating if filters used for white cell reduction of blood components can be used as a source of hemopoietic progenitors. Methods. We used for this study 50 mL buffy-coats (BC) from routine blood donations from healthy donors. We filtered the BC through polyurethane Imugard III-RC filters (Terumo, Tokyo, Japan), originally developed for white cell reduction of RBC. In 6 studies, which were performed within 24 hours of blood donation, we diluted the BC vol/vol with Plasmalyte-A (PL, Baxter, Deerfield, IL), primed the filter with PL, filtered the diluted BC by gravity at RT and rinsed the filter with 50 mL of PL. We then flushed vigorously the filler from outlet to inlet with 350 mL of PL and retrieved the cells in a transfer bag. We finally determined the percent recovery (final versus initial counts per unit) of mononuclear cells (MNC) by a Sismex 9000 hematology counter, the number of CD34+ cells by flow cytometry and of CFU-GM by 2-week culture in methylcellulose. Results. Pre-filtralion BC values/unit were: MNC 770±261×106; CD34+ cells 2.4±1.9×106; CFU-GM 91±73×103. Recoveries after elution were: MNC 93.5±20.7{\%}; CD34+ cells 73.5±13.9{\%}; CFU-GM 96.8±27.1{\%}. Conclusions. A proportion of hemopoietic progenitors present in BC from routine donations can be collected after BC filtration through a commercial white cell reduction filter by filter elution with a crystalloid solution suitable for human use. Our preliminary observations suggest that commercial white cell reduction filters may be a useful tool for collection and retrieval of hemopoietic progenitors from blood components.",
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AU - Rebulla, P.

AU - Sabbioneda, L.

AU - De Bernardi, N.

AU - Lazzari, L.

AU - Porretti, L.

AU - Puglisi, G.

AU - Lecchi, L.

AU - Sirchia, G.

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N2 - Purpose. We were interested in evaluating if filters used for white cell reduction of blood components can be used as a source of hemopoietic progenitors. Methods. We used for this study 50 mL buffy-coats (BC) from routine blood donations from healthy donors. We filtered the BC through polyurethane Imugard III-RC filters (Terumo, Tokyo, Japan), originally developed for white cell reduction of RBC. In 6 studies, which were performed within 24 hours of blood donation, we diluted the BC vol/vol with Plasmalyte-A (PL, Baxter, Deerfield, IL), primed the filter with PL, filtered the diluted BC by gravity at RT and rinsed the filter with 50 mL of PL. We then flushed vigorously the filler from outlet to inlet with 350 mL of PL and retrieved the cells in a transfer bag. We finally determined the percent recovery (final versus initial counts per unit) of mononuclear cells (MNC) by a Sismex 9000 hematology counter, the number of CD34+ cells by flow cytometry and of CFU-GM by 2-week culture in methylcellulose. Results. Pre-filtralion BC values/unit were: MNC 770±261×106; CD34+ cells 2.4±1.9×106; CFU-GM 91±73×103. Recoveries after elution were: MNC 93.5±20.7%; CD34+ cells 73.5±13.9%; CFU-GM 96.8±27.1%. Conclusions. A proportion of hemopoietic progenitors present in BC from routine donations can be collected after BC filtration through a commercial white cell reduction filter by filter elution with a crystalloid solution suitable for human use. Our preliminary observations suggest that commercial white cell reduction filters may be a useful tool for collection and retrieval of hemopoietic progenitors from blood components.

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