Retrospective evaluation of DNA ploidy of hepatocarcinoma on cytologic samples

Pio Zeppa, Giulio Benincasa, Giancarlo Troncone, Antonio Lucariello, Assunta Zabatta, Beatrix Cochand-Priollet, Franco Fulciniti, Antonio Vetrani, Gaetano De Rosa, Lucio Palombini

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DNA ploidy was evaluated by image cytometry in a series of 84 hepatocellular carcinomas diagnosed by fine-needle aspiration biopsy. In the series were included eight cases originally diagnosed as suspect and reclassified as well-differentiated hepatocarcinoma. The study was retrospectively performed on Papanicolaou-destained, Feulgen-restained smears. The 5c exceeding rate and the visual interpretation of the corresponding histograms were evaluated and compared with size of the tumors, serum α-fetoprotein values, hepatic functional staging, and patient survival. Sixty-eight cases were aneuploid and 16 euploid (9 diploid and 7 polyploid). Four of the eight cytologically suspect cases were aneuploid. Statistical analysis showed an association between size and cytologic grading, 5c exceeding rate and cytologic grading, and between aneuploidy and multiple tumors; in a Cox multivariate DNA content analysis, aneuploidy and multiple tumors were the two prognostically significant variables. DNA ploidy evaluation by static cytometry of hepatic tumors may be useful in the diagnosis on cytologic samples and could represent an independent prognostic parameter in predicting the survival outcome of patients with hepatocellular carcinoma.

Original languageEnglish
Pages (from-to)323-329
Number of pages7
JournalDiagnostic Cytopathology
Issue number5
Publication statusPublished - Nov 1998


  • Cytology
  • DNA ploidy
  • Hepatocarcinoma

ASJC Scopus subject areas

  • Anatomy

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    Zeppa, P., Benincasa, G., Troncone, G., Lucariello, A., Zabatta, A., Cochand-Priollet, B., Fulciniti, F., Vetrani, A., De Rosa, G., & Palombini, L. (1998). Retrospective evaluation of DNA ploidy of hepatocarcinoma on cytologic samples. Diagnostic Cytopathology, 19(5), 323-329.<323::AID-DC2>3.0.CO;2-Y