Retroviral-mediated transfer and expression of hepatitis B e antigen in human primary skin fibroblasts and Esptein-Barr virus-transformed B lymphocytes

A. K. Raney, D. R. Milich, J. L. Hughes, J. Sorge, F. V. Chisari, M. U. Mondelli, A. McLachlan

Research output: Contribution to journalArticle

Abstract

Previously, an amphotropic retroviral expression system coding for the neomycin resistance gene was developed and used to synthesize hepatitis B e antigen (HBeAg) and hepatitis B core/e antigen (HBc/eAg) in transfected mouse NIH 3T3 fibroblasts (A. McLachlan et al., 1987, J. Virol. 61, 683-692). In the present study, these transfected cell lines were infected with a helper amphotropic murine leukemia virus resulting in the production of infectious recombinant retrovirus. The recombinant retrovirus was examined for its capacity to transmit resistance to the antibiotic, G418, and to express hepatitis B virus antigens in mouse NIH 3T3 fibroblasts, human primary skin fibroblasts, and Epstein-Barr virus (EBV)-transformed B lymphocytes. A mouse NIH 3T3 fibroblast clone was generated which produced recombinant retrovirus with the capacity to transmit HBeAg expression to these murine and human cell lines. In contrast, it was not possible to transmit HBc/eAg synthesis efficiently to these cell lines by recombinant retroviral infection. The difference between the efficiencies of transmission of HBeAg and HBc/eAg expression by recombinant retroviral-mediated infection was not predicted as the expression vector coding for HBc/eAg synthesis differs only by the deletion of approximately 90 nucleotides of HBV DNA sequence from the vector coding for HBeAg synthesis.

Original languageEnglish
Pages (from-to)31-39
Number of pages9
JournalVirology
Volume168
Issue number1
DOIs
Publication statusPublished - 1989

Fingerprint

Hepatitis B e Antigens
B-Lymphocytes
Fibroblasts
Viruses
Hepatitis B Core Antigens
Skin
Retroviridae
Cell Line
Hepatitis B Antigens
Murine Leukemia Viruses
Neomycin
Microbial Drug Resistance
Infection
Human Herpesvirus 4
Hepatitis B virus
Nucleotides
Clone Cells

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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Retroviral-mediated transfer and expression of hepatitis B e antigen in human primary skin fibroblasts and Esptein-Barr virus-transformed B lymphocytes. / Raney, A. K.; Milich, D. R.; Hughes, J. L.; Sorge, J.; Chisari, F. V.; Mondelli, M. U.; McLachlan, A.

In: Virology, Vol. 168, No. 1, 1989, p. 31-39.

Research output: Contribution to journalArticle

Raney, A. K. ; Milich, D. R. ; Hughes, J. L. ; Sorge, J. ; Chisari, F. V. ; Mondelli, M. U. ; McLachlan, A. / Retroviral-mediated transfer and expression of hepatitis B e antigen in human primary skin fibroblasts and Esptein-Barr virus-transformed B lymphocytes. In: Virology. 1989 ; Vol. 168, No. 1. pp. 31-39.
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