Retroviral microarray-based platform on nanostructured TiO2 for functional genomics and drug discovery

Roberta Carbone, Luca Giorgetti, Andrea Zanardi, Ida Marangi, Elisabetta Chierici, Gero Bongiorno, Francesca Fiorentini, Mario Faretta, Paolo Piseri, Pier Giuseppe Pelicci, Paolo Milani

Research output: Contribution to journalArticle

Abstract

Living-cell microarrays are powerful tools for functional genomics and drug discovery. However, despite several attempts to improve this technology, it is still a challenge to obtain microarrays of cells efficiently overexpressing or downregulating specific genes to address complex phenotypes. Here, we present a cell-based microarray for phenotype screening on primary and cancer cells based on the localized reverse infection by retroviruses. Viral vectors are immobilized on a nanostructured titanium dioxide (ns-TiO2) film obtained by depositing a supersonic beam of titania clusters on a glass substrate. We validated the retroviral cell array by overexpression of GFP reporter genes in primary and cancer cells, and by RNA interference of p53 in primary cells by analyzing effects in cell growth. We demonstrate that ns-TiO2 retroviral arrays are an enabling tool for the study of gene function of families of genes for complex phenotypes and for the identification of novel drug targets.

Original languageEnglish
Pages (from-to)2244-2253
Number of pages10
JournalBiomaterials
Volume28
Issue number13
DOIs
Publication statusPublished - May 2007

Keywords

  • Cell-based microarrays
  • Nanostructured titanium oxide

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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    Carbone, R., Giorgetti, L., Zanardi, A., Marangi, I., Chierici, E., Bongiorno, G., Fiorentini, F., Faretta, M., Piseri, P., Pelicci, P. G., & Milani, P. (2007). Retroviral microarray-based platform on nanostructured TiO2 for functional genomics and drug discovery. Biomaterials, 28(13), 2244-2253. https://doi.org/10.1016/j.biomaterials.2006.12.026