retSDR1, a short-chain retinol dehydrogenase/reductase, is retinoic acid-inducible and frequently deleted in human neuroblastoma cell lines

Fabio Cerignoli, Xiaojia Guo, Beatrice Cardinali, Christian Rinaldi, Jessica Casaletto, Luigi Frati, Isabella Screpanti, Lorraine J. Gudas, Alberto Gulino, Carol J. Thiele, Giuseppe Giannini

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Abstract

Vitamin A is required for a number of developmental processes and for the homeostatic maintenance of several adult differentiated tissues and organs. In human neuroblastoma (NB) cells as well as some other tumor types, pharmacological doses of retinoids are able to control growth and induce differentiation in vitro and in vivo. In a search for new genes that are regulated by retinoids and that contribute to the biological effects retinoids have on NB cells, we have isolated five differentially expressed transcripts. Here we report on the characterization of one of them (DD83.1) in NB cell lines. DD83.1 is identical to the human retSDR1, a short chain dehydrogenase/reductase that is thought to regenerate retinol from retinal in the visual cycle. Its expression is strongly, but differently, regulated by retinoids in NB cell lines, and it is widely expressed in human tissues, which suggests that it is involved in a more general retinol metabolic pathway. Both the retinoic acid-dependent and the exogenous expression of retSDR1 in SK-N-AS cells induce the accumulation of retinyl esters, which indicates that it is involved in generating storage forms of retinol in tissues exposed to physiological retinol concentrations. We also show that the human retSDR1 gene, which maps on chromosome 1p36.1, is contained in the DNA fragment deleted in many NB cell lines bearing MYCN amplification but is conserved in a cell line with a small 1p deletion and normal MYCN. Our observations suggest that retSDR1 is a novel regulator of vitamin A metabolism and that its frequent deletion in NB cells bearing MYCN amplification could compromise the sensitivity of those cells to retinol, thereby contributing to cancer development and progression.

Original languageEnglish
Pages (from-to)1196-1204
Number of pages9
JournalCancer Research
Volume62
Issue number4
Publication statusPublished - 2002

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Tretinoin
Vitamin A
Neuroblastoma
Oxidoreductases
Cell Line
Retinoids
Metabolic Networks and Pathways
alcohol dehydrogenase (NAD(P)+)
retinol dehydrogenase
Genes
Neoplasms
Esters
Chromosomes
Maintenance
Pharmacology
DNA
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Cerignoli, F., Guo, X., Cardinali, B., Rinaldi, C., Casaletto, J., Frati, L., ... Giannini, G. (2002). retSDR1, a short-chain retinol dehydrogenase/reductase, is retinoic acid-inducible and frequently deleted in human neuroblastoma cell lines. Cancer Research, 62(4), 1196-1204.

retSDR1, a short-chain retinol dehydrogenase/reductase, is retinoic acid-inducible and frequently deleted in human neuroblastoma cell lines. / Cerignoli, Fabio; Guo, Xiaojia; Cardinali, Beatrice; Rinaldi, Christian; Casaletto, Jessica; Frati, Luigi; Screpanti, Isabella; Gudas, Lorraine J.; Gulino, Alberto; Thiele, Carol J.; Giannini, Giuseppe.

In: Cancer Research, Vol. 62, No. 4, 2002, p. 1196-1204.

Research output: Contribution to journalArticle

Cerignoli, F, Guo, X, Cardinali, B, Rinaldi, C, Casaletto, J, Frati, L, Screpanti, I, Gudas, LJ, Gulino, A, Thiele, CJ & Giannini, G 2002, 'retSDR1, a short-chain retinol dehydrogenase/reductase, is retinoic acid-inducible and frequently deleted in human neuroblastoma cell lines', Cancer Research, vol. 62, no. 4, pp. 1196-1204.
Cerignoli, Fabio ; Guo, Xiaojia ; Cardinali, Beatrice ; Rinaldi, Christian ; Casaletto, Jessica ; Frati, Luigi ; Screpanti, Isabella ; Gudas, Lorraine J. ; Gulino, Alberto ; Thiele, Carol J. ; Giannini, Giuseppe. / retSDR1, a short-chain retinol dehydrogenase/reductase, is retinoic acid-inducible and frequently deleted in human neuroblastoma cell lines. In: Cancer Research. 2002 ; Vol. 62, No. 4. pp. 1196-1204.
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AU - Cardinali, Beatrice

AU - Rinaldi, Christian

AU - Casaletto, Jessica

AU - Frati, Luigi

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AU - Thiele, Carol J.

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N2 - Vitamin A is required for a number of developmental processes and for the homeostatic maintenance of several adult differentiated tissues and organs. In human neuroblastoma (NB) cells as well as some other tumor types, pharmacological doses of retinoids are able to control growth and induce differentiation in vitro and in vivo. In a search for new genes that are regulated by retinoids and that contribute to the biological effects retinoids have on NB cells, we have isolated five differentially expressed transcripts. Here we report on the characterization of one of them (DD83.1) in NB cell lines. DD83.1 is identical to the human retSDR1, a short chain dehydrogenase/reductase that is thought to regenerate retinol from retinal in the visual cycle. Its expression is strongly, but differently, regulated by retinoids in NB cell lines, and it is widely expressed in human tissues, which suggests that it is involved in a more general retinol metabolic pathway. Both the retinoic acid-dependent and the exogenous expression of retSDR1 in SK-N-AS cells induce the accumulation of retinyl esters, which indicates that it is involved in generating storage forms of retinol in tissues exposed to physiological retinol concentrations. We also show that the human retSDR1 gene, which maps on chromosome 1p36.1, is contained in the DNA fragment deleted in many NB cell lines bearing MYCN amplification but is conserved in a cell line with a small 1p deletion and normal MYCN. Our observations suggest that retSDR1 is a novel regulator of vitamin A metabolism and that its frequent deletion in NB cells bearing MYCN amplification could compromise the sensitivity of those cells to retinol, thereby contributing to cancer development and progression.

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