Reversibility of acute cyclosporin nephrotoxicity by dopamine. Micropuncture study in the rat

M. Sabbatini, C. Esposito, L. De Nicola, F. Uccello, M. Altomonte, G. Conte, A. Dal Canton, V. E. Andreucci

Research output: Contribution to journalArticle

Abstract

The ability of dopamine to neutralise the effects acutely induced by cyclosporin (CsA) on glomerular dynamics was evaluated in four groups of female Munich-Wistar rats, prepared for micropuncture: group I (n = 9), normal rats receiving saline as placebo; group II (n= 10), rats treated with CsA (20mg/kg b.w. in 1 h); group III (n = 8) rats treated with CsA, as in group II, and then with vasodilating doses of dopamine (1.2-2.0 ug/100 g b.w./min in continuous intravenous infusion); group IV (« = 7), rats administered Cremo-phore EL, the vehicle of CsA, in corresponding doses. Single nephron GFR (SNGFR), glomerular plasma flow (GPF), afferent and efferent arteriole resistances (R, and Re, respectively), SN filtration fraction (SNFF), ultrafiltration coefficient (Kf) were measured. Body weight, blood pressure and haematocrit were similar in the four groups. GFR was significantly reduced in group II (0.83±0.08ml/min vs 1.29±0.01 in group I, 1.46 ±0.25 in group III, and 1.40 ±0.09 in group IV, P <0.05 vs all the groups), while no statistical difference was detected in urinary volume. SNGFR was significantly reduced in group II vs group I (18.7+ 1.8nl/min vs 30.6±1.3, -5 vs 2.39±0.22, e: 3.25±0.53 vs 1.59±0.16, Pa and Re were, in fact, significantly reduced vs groups II (R„: 2.31 ±0.25 dyne/sec per cm "5, Pe: 1.46 ± 0.22, P<0.01) determining the return of GPF to normal values (106.2 ±9.7 nl/min, t and by reducing Kf; (b) this effect is not due to Cremophore EL; (c) dopamine is effective in reversing completely the adverse haemodynamic effects acutely induced by CsA.

Original languageEnglish
Pages (from-to)327-333
Number of pages7
JournalNephrology Dialysis Transplantation
Volume4
Issue number5
DOIs
Publication statusPublished - 1989

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Keywords

  • Cyclosporin
  • Dopamine
  • Micropuncture
  • Nephrotoxicity

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

Sabbatini, M., Esposito, C., De Nicola, L., Uccello, F., Altomonte, M., Conte, G., Dal Canton, A., & Andreucci, V. E. (1989). Reversibility of acute cyclosporin nephrotoxicity by dopamine. Micropuncture study in the rat. Nephrology Dialysis Transplantation, 4(5), 327-333. https://doi.org/10.1093/oxfordjournals.ndt.a091885