Reversible effect of MR and ELF magnetic fields (0.5 T and 0.5 mT) on human lymphocyte activation patterns

Sergio Salerno, Carmela La Mendola, Antonio Lo Casto, Giuseppe Mamone, Nadia Caccamo, Adelfio E. Cardinale, Alfredo Salerno

Research output: Contribution to journalArticle

Abstract

Purpose: The aim of this study was to investigate the effects of magnetic fields (MF) of different intensity generated by a magnetic resonance (MR) unit (0.5 Tesla) and a double cylindrical coil (0.5 mTesla) on human CD4+ T cell lines. Materials and methods: CD4+ T cells were exposed for two hours under isothermal conditions (37 ± 0.5°C) to the above mentioned MF; a control group was provided for each exposed sample. After exposure, the samples were analysed in the laboratory for the following endpoints: Release of cytokines, expression of surface markers, cell proliferation and levels of cytosolic free-calcium. Results: Exposure to MF for 2 h and subsequent in vitro stimulation in the presence of the appropriate mitogen, caused a decrease of interferon-γ production, a decrease of cell proliferation, a decrease of expression of CD25 and a decrease of cytosolic free calcium concentration in exposed CD4+ T cell lines. Data obtained, were statistically significant when evaluated after 24 h of in vitro culture, but were not significant, for both types of MF, when the experimental groups were analysed after prolonged in vitro culture. Conclusion: These results indicate that static magnetic fields (SMF) can give rise to transient biological effects on T lymphocytes and the present system is a sensitive model for understanding the effects of MF on the immune system.

Original languageEnglish
Pages (from-to)77-85
Number of pages9
JournalInternational Journal of Radiation Biology
Volume82
Issue number2
DOIs
Publication statusPublished - Feb 2006

Fingerprint

Lymphocytes
lymphocytes
Magnetic resonance
Magnetic Fields
magnetic fields
lymphocyte proliferation
Lymphocyte Activation
magnetic resonance
Magnetic Resonance Spectroscopy
T-cells
Chemical activation
activation
Magnetic fields
T-lymphocytes
T-Lymphocytes
Cell proliferation
cultured cells
in vitro culture
calcium
Calcium

Keywords

  • Cell proliferation
  • Cytokines
  • Lymphocytes
  • Magnetic fields

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Reversible effect of MR and ELF magnetic fields (0.5 T and 0.5 mT) on human lymphocyte activation patterns. / Salerno, Sergio; La Mendola, Carmela; Lo Casto, Antonio; Mamone, Giuseppe; Caccamo, Nadia; Cardinale, Adelfio E.; Salerno, Alfredo.

In: International Journal of Radiation Biology, Vol. 82, No. 2, 02.2006, p. 77-85.

Research output: Contribution to journalArticle

Salerno, Sergio ; La Mendola, Carmela ; Lo Casto, Antonio ; Mamone, Giuseppe ; Caccamo, Nadia ; Cardinale, Adelfio E. ; Salerno, Alfredo. / Reversible effect of MR and ELF magnetic fields (0.5 T and 0.5 mT) on human lymphocyte activation patterns. In: International Journal of Radiation Biology. 2006 ; Vol. 82, No. 2. pp. 77-85.
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