Reversine, a novel Aurora kinases inhibitor, inhibits colony formation of human acute myeloid leukemia cells

Anna Morena D'Alise, Giovanni Amabile, Mariangela Iovino, Francesco Paolo Di Giorgio, Marta Bartiromo, Fabio Sessa, Fabrizio Villa, Andrea Musacchio, Riccardo Cortese

Research output: Contribution to journalArticle

Abstract

The demonstration that the small synthetic molecule reversine [2-(4-morpholinoanilino)-N6-cyclohexyladenine] promotes the dedifferentiation of committed cells into multipotent progenitor-type cells has raised hopes on the exploitation of this small chemical tool for the generation of stem cells. Here, we show that reversine causes a failure in cytokinesis and induces polyploidization. These effects of reversine are due to the inhibition of Aurora A and B, two related kinases that are implicated in several aspects of mitosis and that are frequently amplified and overexpressed in human tumors. Reversine inhibits the phosphorylation of histone H3, a direct downstream target of Aurora kinases. Similarly to the Aurora kinase inhibitor VX-680, which has recently entered phase II clinical trials for cancer treatment, reversine inhibited colony formation of leukemic cells from patients with acute myeloid leukemia but was significantly less toxic than VX-680 on cells from healthy donors. The crystal structure of the reversine-Aurora B kinase complex shows that reversine is a novel class of ATP-competitive Aurora kinase inhibitors. Thus, although our studies raise serious doubts on the application of reversine in regenerative medicine, they support the paradigm that reversine might be a useful agent in cancer chemotherapy.

Original languageEnglish
Pages (from-to)1140-1149
Number of pages10
JournalMolecular Cancer Therapeutics
Volume7
Issue number5
DOIs
Publication statusPublished - 2008

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Drug Discovery
  • Pharmacology

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    D'Alise, A. M., Amabile, G., Iovino, M., Di Giorgio, F. P., Bartiromo, M., Sessa, F., Villa, F., Musacchio, A., & Cortese, R. (2008). Reversine, a novel Aurora kinases inhibitor, inhibits colony formation of human acute myeloid leukemia cells. Molecular Cancer Therapeutics, 7(5), 1140-1149. https://doi.org/10.1158/1535-7163.MCT-07-2051