Reversine increases multipotent human mesenchymal cells differentiation potential.

E. Conforti, E. Arrigoni, M. Piccoli, S. Lopa, L. de Girolamo, A. Ibatici, A. Di Matteo, G. Tettamanti, A. T. Brini, L. Anastasia

Research output: Contribution to journalArticle

Abstract

Among different human stem cell sources, adult mesenchymal stem cells from bone marrow (BMSCs), and more recently from adipose tissues (ASCs), have shown their capability to differentiate into a variety of different cell types, including osteoblasts, adipocytes, and muscle cells. However, mesenchymal stem cell differentiation toward certain cell types (including skeletal and cardiac muscle), while shown to be achievable, still suffers of low yields and needs to be greatly improved before any therapeutic application could be foreseen. A possible way of achieving this goal is by using a chemical-pharmacological approach to increase stem cell plasticity. Along this line, we envisioned the possibility of pre-treating BMSCs and ASCs with reversine, a synthetic purine that has been shown to induce adult cells de-differentiation. In the current study we tested reversine effects on both BMSCs and ASCs to increase their differentiation toward osteoblasts, smooth and skeletal muscle cells. Reversine pre-treatment, at very low concentration (50 nM), caused a marked increase in the differentiation yields of both BMSCs and ASCs.

Original languageEnglish
JournalJournal of Biological Regulators and Homeostatic Agents
Volume25
Issue number2 Suppl
Publication statusPublished - Apr 2011

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Cell Differentiation
Osteoblasts
Mesenchymal Stromal Cells
Skeletal Muscle
Stem Cells
Adult Stem Cells
Adipocytes
Muscle Cells
Smooth Muscle Myocytes
Adipose Tissue
Myocardium
Bone Marrow
Pharmacology
2-(4-morpholinoanilino)-6-cyclohexylaminopurine
Therapeutics
Cell Plasticity
purine

ASJC Scopus subject areas

  • Oncology
  • Endocrinology, Diabetes and Metabolism
  • Physiology (medical)
  • Immunology and Allergy
  • Immunology
  • Endocrinology
  • Physiology
  • Cancer Research

Cite this

Reversine increases multipotent human mesenchymal cells differentiation potential. / Conforti, E.; Arrigoni, E.; Piccoli, M.; Lopa, S.; de Girolamo, L.; Ibatici, A.; Di Matteo, A.; Tettamanti, G.; Brini, A. T.; Anastasia, L.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 25, No. 2 Suppl, 04.2011.

Research output: Contribution to journalArticle

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AU - Ibatici, A.

AU - Di Matteo, A.

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