Reversine-treated fibroblasts acquire myogenic competence in vitro and in regenerating skeletal muscle

Luigi Anastasia, Maurilio Sampaolesi, Nadia Papini, Diego Oleari, Giuseppe Lamorte, Cristina Tringali, Eugenio Monti, Daniela Galli, Guido Tettamanti, Giulio Cossu, Bruno Venerando

Research output: Contribution to journalArticle

Abstract

Stem cells hold a great potential for the regeneration of damaged tissues in cardiovascular or musculoskeletal diseases. Unfortunately, problems such as limited availability, control of cell fate, and allograft rejection need to be addressed before therapeutic applications may become feasible. Generation of multipotent progenitors from adult differentiated cells could be a very attractive alternative to the limited in vitro self-renewal of several types of stem cells. In this direction, a recently synthesized unnatural purine, named reversine, has been proposed to induce reversion of adult cells to a multipotent state, which could be then converted into other cell types under appropriate stimuli. Our study suggests that reversine treatment transforms primary murine and human dermal fibroblasts into myogenic-competent cells both in vitro and in vivo. Moreover, this is the first study to demonstrate that plasticity changes arise in primary mouse and human cells following reversine exposure.

Original languageEnglish
Pages (from-to)2042-2051
Number of pages10
JournalCell Death and Differentiation
Volume13
Issue number12
DOIs
Publication statusPublished - Dec 2006

ASJC Scopus subject areas

  • Cell Biology

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    Anastasia, L., Sampaolesi, M., Papini, N., Oleari, D., Lamorte, G., Tringali, C., Monti, E., Galli, D., Tettamanti, G., Cossu, G., & Venerando, B. (2006). Reversine-treated fibroblasts acquire myogenic competence in vitro and in regenerating skeletal muscle. Cell Death and Differentiation, 13(12), 2042-2051. https://doi.org/10.1038/sj.cdd.4401958