Abstract
More than a decade of work has been devoted to engineering cartilage for articular surface repair. This review covers the use of fibrin gel polymer as an injectable scaffold for generating new cartilage matrix from isolated articular chondrocytes beginning with studies in mice and culminating in an applied study in swine joints. These studies began with developing a formulation of fibrin that was injectable and promoted cartilage matrix formation. Subsequent studies addressed the problems of volume loss after the scaffolds were placed in vivo by adding lyophilized cartilage matrix. Additional studies focused on the ability of isolated chondrocytes to heal and repair cartilage in a model that could be biomechanically tested. In conclusion, this series of studies demonstrated that fibrin gel is a suitable polymer gel for generating new cartilage matrix from articular chondrocytes. The new matrix is capable of forming mechanical bonds between cartilage disks and can lead to healing and integration. Armed with these results, implantation of fibrin-cell constructs into defects in swine knees showed new cartilage formation and filling of the defects. Continuing work in these models with fibrin and other polymerizable hydrogels could result in a suitable cell-based therapy for articular cartilage lesions.
| Original language | English |
|---|---|
| Pages (from-to) | 1151-1168 |
| Number of pages | 18 |
| Journal | Tissue Engineering |
| Volume | 12 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 2006 |
Fingerprint
ASJC Scopus subject areas
- Biophysics
- Cell Biology
- Biotechnology
Cite this
Review of injectable cartilage engineering using fibrin gel in mice and swine models. / Peretti, Giuseppe M.; Xu, Jian Wei; Bonassar, Lawrence J.; Kirchhoff, Carl Hendrick; Yaremchuk, Michael J.; Randolph, Mark A.
In: Tissue Engineering, Vol. 12, No. 5, 05.2006, p. 1151-1168.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Review of injectable cartilage engineering using fibrin gel in mice and swine models
AU - Peretti, Giuseppe M.
AU - Xu, Jian Wei
AU - Bonassar, Lawrence J.
AU - Kirchhoff, Carl Hendrick
AU - Yaremchuk, Michael J.
AU - Randolph, Mark A.
PY - 2006/5
Y1 - 2006/5
N2 - More than a decade of work has been devoted to engineering cartilage for articular surface repair. This review covers the use of fibrin gel polymer as an injectable scaffold for generating new cartilage matrix from isolated articular chondrocytes beginning with studies in mice and culminating in an applied study in swine joints. These studies began with developing a formulation of fibrin that was injectable and promoted cartilage matrix formation. Subsequent studies addressed the problems of volume loss after the scaffolds were placed in vivo by adding lyophilized cartilage matrix. Additional studies focused on the ability of isolated chondrocytes to heal and repair cartilage in a model that could be biomechanically tested. In conclusion, this series of studies demonstrated that fibrin gel is a suitable polymer gel for generating new cartilage matrix from articular chondrocytes. The new matrix is capable of forming mechanical bonds between cartilage disks and can lead to healing and integration. Armed with these results, implantation of fibrin-cell constructs into defects in swine knees showed new cartilage formation and filling of the defects. Continuing work in these models with fibrin and other polymerizable hydrogels could result in a suitable cell-based therapy for articular cartilage lesions.
AB - More than a decade of work has been devoted to engineering cartilage for articular surface repair. This review covers the use of fibrin gel polymer as an injectable scaffold for generating new cartilage matrix from isolated articular chondrocytes beginning with studies in mice and culminating in an applied study in swine joints. These studies began with developing a formulation of fibrin that was injectable and promoted cartilage matrix formation. Subsequent studies addressed the problems of volume loss after the scaffolds were placed in vivo by adding lyophilized cartilage matrix. Additional studies focused on the ability of isolated chondrocytes to heal and repair cartilage in a model that could be biomechanically tested. In conclusion, this series of studies demonstrated that fibrin gel is a suitable polymer gel for generating new cartilage matrix from articular chondrocytes. The new matrix is capable of forming mechanical bonds between cartilage disks and can lead to healing and integration. Armed with these results, implantation of fibrin-cell constructs into defects in swine knees showed new cartilage formation and filling of the defects. Continuing work in these models with fibrin and other polymerizable hydrogels could result in a suitable cell-based therapy for articular cartilage lesions.
UR - http://www.scopus.com/inward/record.url?scp=33745798853&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745798853&partnerID=8YFLogxK
U2 - 10.1089/ten.2006.12.1151
DO - 10.1089/ten.2006.12.1151
M3 - Article
VL - 12
SP - 1151
EP - 1168
JO - Tissue Engineering
JF - Tissue Engineering
SN - 1076-3279
IS - 5
ER -