ReViMS: Software tool for estimating the volumes of 3-D multicellular spheroids imaged using a light sheet fluorescence microscope

Filippo Piccinini, Anna Tesei, Michele Zanoni, Alessandro Bevilacqua

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cancer 3-D spheroids are widely used to test drugs and radiotherapy treatments. These 3-D cell clusters range from tens to hundreds of micrometers in size, with shapes that typically differ from a perfect sphere. Change in spheroid volume is one of the most important parameters for evaluating treatment efficacy, and using light sheet fluorescence microscopes (LSFM), optical sections of samples in that size range can be obtained. However, there remains a lack of validated methods for quantifying the volumes of 3-D multicellular aggregates. Here, we present Reconstruction and Visualization from Multiple Sections (ReViMS), an open-source, user-friendly software for automatically segmenting z-stacks of fluorescence images and estimating the volumes of 3-D multicellular spheroids. To assess the precision and accuracy of the volume estimates obtained with ReViMS, we used several cancer spheroids imaged with LSFM. Both the precision and accuracy were >95%, demonstrating the effectiveness of ReViMS.

Original languageEnglish
Pages (from-to)227-229
Number of pages3
JournalBioTechniques
Volume63
Issue number5
Publication statusPublished - Nov 1 2017

Fingerprint

Cellular Spheroids
Microscopes
Software
Visualization
Fluorescence
Light
Somatostatin-Secreting Cells
Radiotherapy
Sample Size
Neoplasms
Pharmaceutical Preparations
Therapeutics

Keywords

  • Cancer 3D models
  • Image processing
  • Light sheet imaging
  • Microscopy
  • Oncology
  • Opensource software
  • Tumor volume

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

ReViMS : Software tool for estimating the volumes of 3-D multicellular spheroids imaged using a light sheet fluorescence microscope. / Piccinini, Filippo; Tesei, Anna; Zanoni, Michele; Bevilacqua, Alessandro.

In: BioTechniques, Vol. 63, No. 5, 01.11.2017, p. 227-229.

Research output: Contribution to journalArticle

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