Rewiring carbohydrate catabolism differentially affects survival of pancreatic cancer cell lines with diverse metabolic profiles

Tiziana Tataranni, Francesca Agriesti, Vitalba Ruggieri, Carmela Mazzoccoli, Vittorio Simeon, Ilaria Laurenzana, Rosella Scrima, Valerio Pazienza, Nazzareno Capitanio, Claudia Piccoli

Research output: Contribution to journalArticle

Abstract

An increasing body of evidence suggests that targeting cellular metabolism represents a promising effective approach to treat pancreatic cancer, overcome chemoresistance and ameliorate patient's prognosis and survival. In this study, following whole-genome expression analysis, we selected two pancreatic cancer cell lines, PANC-1 and BXPC-3, hallmarked by distinct metabolic profiles with specific concern to carbohydrate metabolism. Functional comparative analysis showed that BXPC-3 displayed a marked deficit of the mitochondrial respiratory and oxidative phosphorylation activity and a higher production of reactive oxygen species and a reduced NAD+/NADH ratio, indicating their bioenergetic reliance on glycolysis and a different redox homeostasis as compared to PANC-1. Both cell lines were challenged to rewire their metabolism by substituting glucose with galactose as carbon source, a condition inhibiting the glycolytic flux and fostering full oxidation of the sugar carbons. The obtained data strikingly show that the mitochondrial respiration-impaired-BXPC-3 cell line was unable to sustain the metabolic adaptation required by glucose deprivation/substitution, thereby resulting in a G2\M cell cycle shift, unbalance of the redox homeostasis, apoptosis induction. Conversely, the mitochondrial respiration-competent-PANC-1 cell line did not show clear evidence of cell sufferance. Our findings provide a strong rationale to candidate metabolism as a promising target for cancer therapy. Defining the metabolic features at time of pancreatic cancer diagnosis and likely of other tumors, appears to be crucial to predict the responsiveness to therapeutic approaches or coadjuvant interventions affecting metabolism.

Original languageEnglish
Pages (from-to)41265-41281
Number of pages17
JournalOncotarget
Volume8
Issue number25
DOIs
Publication statusPublished - Jun 20 2017

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Metabolome
Pancreatic Neoplasms
Carbohydrates
Cell Line
Survival
NAD
Oxidation-Reduction
Respiration
Homeostasis
Carbon
Glucose
Foster Home Care
Oxidative Phosphorylation
Carbohydrate Metabolism
Glycolysis
Galactose
Energy Metabolism
Reactive Oxygen Species
Neoplasms
Cell Cycle

Keywords

  • Journal Article

Cite this

Tataranni, T., Agriesti, F., Ruggieri, V., Mazzoccoli, C., Simeon, V., Laurenzana, I., ... Piccoli, C. (2017). Rewiring carbohydrate catabolism differentially affects survival of pancreatic cancer cell lines with diverse metabolic profiles. Oncotarget, 8(25), 41265-41281. https://doi.org/10.18632/oncotarget.17172

Rewiring carbohydrate catabolism differentially affects survival of pancreatic cancer cell lines with diverse metabolic profiles. / Tataranni, Tiziana; Agriesti, Francesca; Ruggieri, Vitalba; Mazzoccoli, Carmela; Simeon, Vittorio; Laurenzana, Ilaria; Scrima, Rosella; Pazienza, Valerio; Capitanio, Nazzareno; Piccoli, Claudia.

In: Oncotarget, Vol. 8, No. 25, 20.06.2017, p. 41265-41281.

Research output: Contribution to journalArticle

Tataranni, T, Agriesti, F, Ruggieri, V, Mazzoccoli, C, Simeon, V, Laurenzana, I, Scrima, R, Pazienza, V, Capitanio, N & Piccoli, C 2017, 'Rewiring carbohydrate catabolism differentially affects survival of pancreatic cancer cell lines with diverse metabolic profiles', Oncotarget, vol. 8, no. 25, pp. 41265-41281. https://doi.org/10.18632/oncotarget.17172
Tataranni, Tiziana ; Agriesti, Francesca ; Ruggieri, Vitalba ; Mazzoccoli, Carmela ; Simeon, Vittorio ; Laurenzana, Ilaria ; Scrima, Rosella ; Pazienza, Valerio ; Capitanio, Nazzareno ; Piccoli, Claudia. / Rewiring carbohydrate catabolism differentially affects survival of pancreatic cancer cell lines with diverse metabolic profiles. In: Oncotarget. 2017 ; Vol. 8, No. 25. pp. 41265-41281.
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