RGDS peptide inhibits activation of lymphocytes and adhesion of activated lymphocytes to human umbilical vein endothelial cells in vitro

Ubaldo Pozzetto, Maria Simona Aguzzi, Nicola Maggiano, Enrico Scala, Arnaldo Capelli, Marco Castagneto, Maurizio C. Capogrossi, Franco Citterio, Francesco Serino, Antonio Facchiano

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The Arg-Gly-Asp (RGD) motif is known to mediate cell adhesion to several extracellular matrix components as well as cell-cell interactions. In the present study, we investigated whether the RGDS peptide interferes with cell-cell recognition-based events such as allogeneic activation of PBMC and PBMC adhesion to human umbilical vein endothelial cells (HUVEC). We show here for the first time, to our knowledge, that RGDS significantly inhibits adhesion of activated PBMC to HUVEC; in addition, RODS inhibits PBMC allogenenic activation in human mixed lymphocyte reaction assays. Caspases played a pivotal role in both events, because preventing their activation abolished or strongly reduced the observed inhibitory effect. The RGDS antirecognition effect was strongly increased by pretreatment of HUVEC with RGDS, which affected mostly T lymphocyte adhesion to HUVEC. These results indicate that PBMC allogeneic activation, as well as reciprocal recognition between activated PBMC and endothelial cells, are RGDS-dependent events that occur through a dual effect involving anti-adhesive and caspase-dependent mechanisms. These data suggest a potential role of RGDS in cell-mediated immunity, inflammation and organ transplantation.

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalImmunology and Cell Biology
Volume83
Issue number1
DOIs
Publication statusPublished - Feb 2005

Fingerprint

arginyl-glycyl-aspartyl-serine
Lymphocytes
Endothelial cells
Human Umbilical Vein Endothelial Cells
Lymphocyte Activation
Adhesion
Chemical activation
Caspases
Mixed Lymphocyte Culture Test
Transplantation (surgical)
Organ Transplantation
Cell Adhesion
Cellular Immunity
Cell Communication
Adhesives
T-cells
Cell adhesion
Extracellular Matrix
Endothelial Cells
Inflammation

Keywords

  • Cell-cell interaction
  • Endothelial cell
  • Immune suppression
  • Leucocyte
  • Peptide

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Immunology

Cite this

RGDS peptide inhibits activation of lymphocytes and adhesion of activated lymphocytes to human umbilical vein endothelial cells in vitro. / Pozzetto, Ubaldo; Aguzzi, Maria Simona; Maggiano, Nicola; Scala, Enrico; Capelli, Arnaldo; Castagneto, Marco; Capogrossi, Maurizio C.; Citterio, Franco; Serino, Francesco; Facchiano, Antonio.

In: Immunology and Cell Biology, Vol. 83, No. 1, 02.2005, p. 25-32.

Research output: Contribution to journalArticle

Pozzetto, Ubaldo ; Aguzzi, Maria Simona ; Maggiano, Nicola ; Scala, Enrico ; Capelli, Arnaldo ; Castagneto, Marco ; Capogrossi, Maurizio C. ; Citterio, Franco ; Serino, Francesco ; Facchiano, Antonio. / RGDS peptide inhibits activation of lymphocytes and adhesion of activated lymphocytes to human umbilical vein endothelial cells in vitro. In: Immunology and Cell Biology. 2005 ; Vol. 83, No. 1. pp. 25-32.
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