Rheumatoid synovial fibroblast constitutively express the fibrinolytic pattern of invasive tumor-like cells

Serena Guiducci, Angela Del Rosso, Marina Cinelli, Francesca Margheri, Silvia D'Alessio, Gabriella Fibbi, Marco Matucci Cerinic, Mario Del Rosso

Research output: Contribution to journalArticle

Abstract

Objective: In rheumatoid arthritis (RA) the synovial membrane proliferates and invades the underlying tissues. The cell-associated fibrinolytic system (urokinase-type plasminogen activator, uPA; uPA receptor, uPAR; plasminogen activator inhibitor-type 1, PAI-1) is pivotal in cell invasion and proliferation. For this reason, the expression and the role of such enzymatic system was investigated in synovial fibroblasts (SF) of normal and RA patients. Methods: In SF obtained from RA patients and control subjects, uPA, uPAR and PAI-1 were measured by ELISA of cell lysates and culture medium and by RT-PCR of mRNAs. uPA was also studied by zymography. Proliferation was measured by cell counting and cell invasion with the Boyden chamber. Results: RA-SF over-express uPAR and PAI-1 and are more prone than the normal counterpart to spontaneous and uPA-challenged invasion and proliferation, which are counteracted by antagonists of the fibrinolytic system. Conclusions: RA-SF display the fibrinolytic pattern and behaviour of invasive tumor-like cells. Antagonists of the fibrinolytic system are able to revert growth and invasion of both normal and RA-SF.

Original languageEnglish
Pages (from-to)364-372
Number of pages9
JournalClinical and Experimental Rheumatology
Volume23
Issue number3
Publication statusPublished - May 2005

Fingerprint

Rheumatoid Arthritis
Fibroblasts
Plasminogen Activator Inhibitor 1
Neoplasms
Synovial Membrane
Urokinase-Type Plasminogen Activator
Culture Media
Cell Culture Techniques
Enzyme-Linked Immunosorbent Assay
Cell Proliferation
Polymerase Chain Reaction
Messenger RNA
Growth

Keywords

  • Fibrinolytic system
  • Invasion
  • PAI-1
  • Proliferation
  • Rheumatoid arthritis
  • Synovial fibroblasts
  • uPA
  • uPAR

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Guiducci, S., Del Rosso, A., Cinelli, M., Margheri, F., D'Alessio, S., Fibbi, G., ... Del Rosso, M. (2005). Rheumatoid synovial fibroblast constitutively express the fibrinolytic pattern of invasive tumor-like cells. Clinical and Experimental Rheumatology, 23(3), 364-372.

Rheumatoid synovial fibroblast constitutively express the fibrinolytic pattern of invasive tumor-like cells. / Guiducci, Serena; Del Rosso, Angela; Cinelli, Marina; Margheri, Francesca; D'Alessio, Silvia; Fibbi, Gabriella; Cerinic, Marco Matucci; Del Rosso, Mario.

In: Clinical and Experimental Rheumatology, Vol. 23, No. 3, 05.2005, p. 364-372.

Research output: Contribution to journalArticle

Guiducci, S, Del Rosso, A, Cinelli, M, Margheri, F, D'Alessio, S, Fibbi, G, Cerinic, MM & Del Rosso, M 2005, 'Rheumatoid synovial fibroblast constitutively express the fibrinolytic pattern of invasive tumor-like cells', Clinical and Experimental Rheumatology, vol. 23, no. 3, pp. 364-372.
Guiducci, Serena ; Del Rosso, Angela ; Cinelli, Marina ; Margheri, Francesca ; D'Alessio, Silvia ; Fibbi, Gabriella ; Cerinic, Marco Matucci ; Del Rosso, Mario. / Rheumatoid synovial fibroblast constitutively express the fibrinolytic pattern of invasive tumor-like cells. In: Clinical and Experimental Rheumatology. 2005 ; Vol. 23, No. 3. pp. 364-372.
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