Ribosomal DNA sequencing and phylogenetic analysis of environmental sporothrix schenckii strains

Comparison with clinical isolates

Giuseppe Criseo, Orazio Romeo

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The authors reported the isolation and genetic characterization of Sporothrix schenckii strains from natural environmental samples and commercial amended and garden soils. Twenty-six isolates were recovered and identified as S. schenckii by using both phenotypic and molecular methods. The majority of the strains were isolated from commercial amended and garden soils, indicating that these products represent an important reservoir of the fungus. Sequencing and phylogenetic analysis of the D1-D2 region of the 28S rRNA gene of environmental isolates, including S. schenckii ATCC 10268 and two Italian clinical strains, revealed a degree of difference sufficient to justify the separation of the examined isolates in two principal groups (environmental and clinical). Such separation in two groups is further supported by two well-conserved nucleotide polymorphisms, caused by single-base transitions, in the D1-D2 domain of rDNA. In this study, the presence in nature and/or in commercial products of S. schenckii is discussed. To our knowledge, this is the first study that reports the environmental isolation of S. schenckii from southern Italy.

Original languageEnglish
Pages (from-to)351-358
Number of pages8
JournalMycopathologia
Volume169
Issue number5
DOIs
Publication statusPublished - May 2010

Fingerprint

Sporothrix
Sporothrix schenckii
Ribosomal DNA
DNA Sequence Analysis
ribosomal DNA
Soil
sequence analysis
phylogeny
rRNA Genes
Italy
Single Nucleotide Polymorphism
Fungi
gardens
soil
nucleotides
Gardens
ribosomal RNA
genetic polymorphism
fungi
genes

Keywords

  • 28S rDNA
  • Environmental isolates
  • Phylogenetics
  • Sporothrix schenckii
  • Sporotrichosis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Applied Microbiology and Biotechnology
  • Microbiology
  • veterinary (miscalleneous)

Cite this

Ribosomal DNA sequencing and phylogenetic analysis of environmental sporothrix schenckii strains : Comparison with clinical isolates. / Criseo, Giuseppe; Romeo, Orazio.

In: Mycopathologia, Vol. 169, No. 5, 05.2010, p. 351-358.

Research output: Contribution to journalArticle

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