TY - JOUR
T1 - Ribosome-inactivating proteins depurinate poly(ADP-ribosyl)ated poly(ADP-ribose) polymerase and have transforming activity for 3T3 fibroblasts
AU - Barbieri, Luigi
AU - Brigotti, Maurizio
AU - Perocco, Paolo
AU - Carnicelli, Domenica
AU - Ciani, Marialibera
AU - Mercatali, Laura
AU - Stirpe, Fiorenzo
PY - 2003/3/13
Y1 - 2003/3/13
N2 - It has been known that ribosome-inactivating proteins (RIPs) from plants damage ribosomes by removing adenine from a precise position of rRNA. Subsequently it was observed that all tested RIPs depurinate DNA, and some of them also non-ribosomal RNAs and poly(A), hence the denomination of adenine polynucleotide glycosylases was proposed. We report now that ricin, saporin-L2, saporin-S6, gelonin and momordin depurinate also poly(ADP-ribosyl)ated poly(ADP-ribose) polymerase (auto modified enzyme), an enzyme involved in DNA repair. We observed also that all RIPs but gelonin induce transformation of fibroblasts, possibly as a consequence of damage to DNA and of the altered DNA repair system.
AB - It has been known that ribosome-inactivating proteins (RIPs) from plants damage ribosomes by removing adenine from a precise position of rRNA. Subsequently it was observed that all tested RIPs depurinate DNA, and some of them also non-ribosomal RNAs and poly(A), hence the denomination of adenine polynucleotide glycosylases was proposed. We report now that ricin, saporin-L2, saporin-S6, gelonin and momordin depurinate also poly(ADP-ribosyl)ated poly(ADP-ribose) polymerase (auto modified enzyme), an enzyme involved in DNA repair. We observed also that all RIPs but gelonin induce transformation of fibroblasts, possibly as a consequence of damage to DNA and of the altered DNA repair system.
KW - Adenine polynucleotide glycosylase
KW - Cell transformation
KW - Poly(ADP-ribose) polymerase
KW - Ribosome-inactivating protein
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U2 - 10.1016/S0014-5793(03)00176-5
DO - 10.1016/S0014-5793(03)00176-5
M3 - Article
C2 - 12633875
AN - SCOPUS:0037434897
VL - 538
SP - 178
EP - 182
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-3
ER -