RIP2 regulates growth and differentiation of normal myoblasts and of rhabdomyosarcoma cells

Svenja Ehlers, Tobias Mueck, Stephanie Adams, Lorena Landuzzi, Pier Luigi Lollini, Barbara Munz

Research output: Contribution to journalArticle

Abstract

RIP2 is an important regulator of myoblast proliferation and differentiation. We have previously demonstrated that in the myoblast cell line C2C12 and in primary myoblasts, downregulation of rip2 gene expression is a prerequisite for differentiation. To further study the role of rip genes in myogenesis, we compared expression patterns of rip1-4 in two myoblast cell lines, C2C12 and C2F3, after the induction of differentiation. These two cell lines are derived from the same clonal origin, but differ with respect to their differentiation behaviour: specifically, the differentiation process is slower and more incomplete in C2F3 cells. When analyzing cells up to 4 days after the induction of differentiation, we found no downregulation of rip2 gene expression in C2F3 cells, which might be linked to the low differentiation potential of these cells. In addition, in contrast to C2C12 cells, the rip3 gene was not expressed in C2F3 cells. To further study the role of rip genes in the regulation of myoblast growth and differentiation, we analyzed expression patterns of rip1-4 in rhabdomyosarcoma cell lines. We found that in these cells, rip2 expression was not downregulated after the induction of differentiation. Furthermore, in contrast to normal myoblasts, they did not express the rip3 and rip4 genes. Thus, we focused on the functional role of RIP2 in rhabdomyosarcoma cells. Inhibition of rip2 gene expression in C2C12 and in rhabdomyosarcoma cells using specific siRNAs led to decreased proliferation and promoted the differentiation process of these cells. These data indicate that differential expression of rip genes can be associated with abnormal growth and differentiation behaviour of skeletal myoblasts.

Original languageEnglish
Pages (from-to)163-172
Number of pages10
JournalEuropean Journal of Cell Biology
Volume87
Issue number3
DOIs
Publication statusPublished - Mar 17 2008

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Rhabdomyosarcoma
Myoblasts
Growth
Gene Expression
Cell Line
Down-Regulation
Genes
Cell Differentiation
Skeletal Myoblasts
Muscle Development

Keywords

  • Rhabdomyosarcoma
  • RIP proteins
  • Skeletal muscle

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

RIP2 regulates growth and differentiation of normal myoblasts and of rhabdomyosarcoma cells. / Ehlers, Svenja; Mueck, Tobias; Adams, Stephanie; Landuzzi, Lorena; Lollini, Pier Luigi; Munz, Barbara.

In: European Journal of Cell Biology, Vol. 87, No. 3, 17.03.2008, p. 163-172.

Research output: Contribution to journalArticle

Ehlers, Svenja ; Mueck, Tobias ; Adams, Stephanie ; Landuzzi, Lorena ; Lollini, Pier Luigi ; Munz, Barbara. / RIP2 regulates growth and differentiation of normal myoblasts and of rhabdomyosarcoma cells. In: European Journal of Cell Biology. 2008 ; Vol. 87, No. 3. pp. 163-172.
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