TY - JOUR
T1 - RISC RNA sequencing in the Dorsal Raphè reveals microRNAs regulatory activities associated with behavioral and functional adaptations to chronic stress
AU - Babicola, Lucy
AU - Pietrosanto, Marco
AU - Ielpo, Donald
AU - D'Addario, Sebastian Luca
AU - Cabib, Simona
AU - Ventura, Rossella
AU - Ferlazzo, Fabio
AU - Helmer-Citterich, Manuela
AU - Andolina, Diego
AU - Lo Iacono, Luisa
N1 - Funding Information:
This research was supported by the Italian Ministry of Education, Universities and Research (MIUR), Italy, grant ID: SIR BRBSI14G1HH , and by the Sapienza University of Rome , Italy, grant ID: Ateneo 2018 - RG118164367FF640 .
Publisher Copyright:
© 2020 Elsevier B.V.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/6/1
Y1 - 2020/6/1
N2 - The Dorsal Raphe (DR) is the primary source of serotonergic input in the brain and a center for the homeostatic maintenance of the serotonergic tone. Under repeated stimulation, it can undergo adaptive modifications that alter serotonergic neurotransmission, which can lead to behavioral dysfunction. Post-transcriptional regulation by microRNAs is implicated in these adaptations. However, a global microRNA/target network effect on the DR neuroplasticity has yet to be elucidated. Here we investigate the microRNAs/mRNAs regulatory activity in the mouse DR after a chronic stress experience. First, we assessed the behavioral consequences of repeated restraint stress exposure and the functional adaptations of the DR by measuring the change in acute stress-induced serotonin release. Then, through next generation RNA-Seq of Argonaute2-bound RNA (RISC-Seq) we identified microRNAs and their targets that are associated to the RISC complex of the DR in unstressed and stressed mice. We mapped the potential microRNA/mRNA network within the stress-altered transcripts, uncovering new interactions that contribute to the chronic stress-induced DR modifications.
AB - The Dorsal Raphe (DR) is the primary source of serotonergic input in the brain and a center for the homeostatic maintenance of the serotonergic tone. Under repeated stimulation, it can undergo adaptive modifications that alter serotonergic neurotransmission, which can lead to behavioral dysfunction. Post-transcriptional regulation by microRNAs is implicated in these adaptations. However, a global microRNA/target network effect on the DR neuroplasticity has yet to be elucidated. Here we investigate the microRNAs/mRNAs regulatory activity in the mouse DR after a chronic stress experience. First, we assessed the behavioral consequences of repeated restraint stress exposure and the functional adaptations of the DR by measuring the change in acute stress-induced serotonin release. Then, through next generation RNA-Seq of Argonaute2-bound RNA (RISC-Seq) we identified microRNAs and their targets that are associated to the RISC complex of the DR in unstressed and stressed mice. We mapped the potential microRNA/mRNA network within the stress-altered transcripts, uncovering new interactions that contribute to the chronic stress-induced DR modifications.
KW - Chronic stress
KW - Dorsal Raphe
KW - MicroRNA regulatory activity
KW - Research Domain Criteria
KW - RISC sequencing
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U2 - 10.1016/j.brainres.2020.146763
DO - 10.1016/j.brainres.2020.146763
M3 - Article
C2 - 32169579
AN - SCOPUS:85081672685
VL - 1736
JO - Brain Research
JF - Brain Research
SN - 0006-8993
M1 - 146763
ER -